4GP6

Polynucleotide kinase

4GP6 の概要

• エントリーDOI: 10.2210/pdb4gp6/pdb
• 関連するPDBエントリー: 3TY5 3TY8 3TY9 4DRF 4E6N 4GP7
• 分子名称: Metallophosphoesterase, ADENOSINE-5'-DIPHOSPHATE, MAGNESIUM ION, ... (4 entities in total)
• 機能のキーワード: polynucleotide kinase phosphatase, rna repair, transferase
• 由来する生物種: Clostridium thermocellum
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 39843.56

構造登録者

Wang, L.K.,Das, U.,Smith, P.,Shuman, S. (登録日: 2012-08-20, 公開日: 2012-11-14, 最終更新日: 2024-02-28)

主引用文献

Wang, L.K.,Das, U.,Smith, P.,Shuman, S.
Structure and mechanism of the polynucleotide kinase component of the bacterial Pnkp-Hen1 RNA repair system.
Rna, 18:2277-2286, 2012

PubMed Abstract: Pnkp is the end-healing and end-sealing component of an RNA repair system present in diverse bacteria from many phyla. Pnkp is composed of three catalytic modules: an N-terminal polynucleotide 5'-kinase, a central 2',3' phosphatase, and a C-terminal ligase. Here we report the crystal structure of the kinase domain of Clostridium thermocellum Pnkp bound to ATP•Mg²⁺ (substrate complex) and ADP•Mg²⁺ (product complex). The protein consists of a core P-loop phosphotransferase fold embellished by a distinctive homodimerization module composed of secondary structure elements derived from the N and C termini of the kinase domain. ATP is bound within a crescent-shaped groove formed by the P-loop (¹⁵GSSGSGKST²³) and an overlying helix-loop-helix "lid." The α and β phosphates are engaged by a network of hydrogen bonds from Thr23 and the P-loop main-chain amides; the γ phosphate is anchored by the lid residues Arg120 and Arg123. The P-loop lysine (Lys21) and the catalytic Mg²⁺ bridge the ATP β and γ phosphates. The P-loop serine (Ser22) is the sole enzymic constituent of the octahedral metal coordination complex. Structure-guided mutational analysis underscored the essential contributions of Lys21 and Ser22 in the ATP donor site and Asp38 and Arg41 in the phosphoacceptor site. Our studies suggest a catalytic mechanism whereby Asp38 (as general base) activates the polynucleotide 5'-OH for its nucleophilic attack on the γ phosphorus and Lys21 and Mg²⁺ stabilize the transition state.

PubMed: 23118415
DOI: 10.1261/rna.036061.112

実験手法

X-RAY DIFFRACTION (2.1 Å)