4GN0

De novo phasing of a Hamp-complex using an improved Arcimboldo method

Summary for 4GN0

Entry DOI	10.2210/pdb4gn0/pdb
Descriptor	Hamp domain of AF1503, MAGNESIUM ION (3 entities in total)
Functional Keywords	four helix bundle, hamp domain, transmembrane signalling, signaling protein
Biological source	Archaeoglobus fulgidus
Total number of polymer chains	4
Total formula weight	45583.52
Authors	Hulko, M.,Ursinus, A.,Bar, K.,Martin, J.,Zeth, K.,Lupas, A.N. (deposition date: 2012-08-16, release date: 2013-09-11, Last modification date: 2024-02-28)
Primary citation	Sammito, M.,Millan, C.,Rodriguez, D.D.,de Ilarduya, I.M.,Meindl, K.,De Marino, I.,Petrillo, G.,Buey, R.M.,de Pereda, J.M.,Zeth, K.,Sheldrick, G.M.,Uson, I. Exploiting tertiary structure through local folds for crystallographic phasing. Nat.Methods, 10:1099-1101, 2013 Cited by PubMed Abstract: We describe an algorithm for phasing protein crystal X-ray diffraction data that identifies, retrieves, refines and exploits general tertiary structural information from small fragments available in the Protein Data Bank. The algorithm successfully phased, through unspecific molecular replacement combined with density modification, all-helical, mixed alpha-beta, and all-beta protein structures. The method is available as a software implementation: Borges. PubMed: 24037245 DOI: 10.1038/nmeth.2644 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (1.75 Å)

Structure validation