4GN0

De novo phasing of a Hamp-complex using an improved Arcimboldo method

4GN0 の概要

• エントリーDOI: 10.2210/pdb4gn0/pdb
• 分子名称: Hamp domain of AF1503, MAGNESIUM ION (3 entities in total)
• 機能のキーワード: four helix bundle, hamp domain, transmembrane signalling, signaling protein
• 由来する生物種: Archaeoglobus fulgidus
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 45583.52

構造登録者

Hulko, M.,Ursinus, A.,Bar, K.,Martin, J.,Zeth, K.,Lupas, A.N. (登録日: 2012-08-16, 公開日: 2013-09-11, 最終更新日: 2024-02-28)

主引用文献

Sammito, M.,Millan, C.,Rodriguez, D.D.,de Ilarduya, I.M.,Meindl, K.,De Marino, I.,Petrillo, G.,Buey, R.M.,de Pereda, J.M.,Zeth, K.,Sheldrick, G.M.,Uson, I.
Exploiting tertiary structure through local folds for crystallographic phasing.
Nat.Methods, 10:1099-1101, 2013

PubMed Abstract: We describe an algorithm for phasing protein crystal X-ray diffraction data that identifies, retrieves, refines and exploits general tertiary structural information from small fragments available in the Protein Data Bank. The algorithm successfully phased, through unspecific molecular replacement combined with density modification, all-helical, mixed alpha-beta, and all-beta protein structures. The method is available as a software implementation: Borges.

PubMed: 24037245
DOI: 10.1038/nmeth.2644

実験手法

X-RAY DIFFRACTION (1.75 Å)