4GMP

Crystal structure of enterovirus 71 strain 1095 procapsid

4GMP の概要

• エントリーDOI: 10.2210/pdb4gmp/pdb
• 分子名称: capsid protein VP0, capsid protein VP1, capsid protein VP3 (3 entities in total)
• 機能のキーワード: capsid protein, virus
• 由来する生物種: Human enterovirus 71; 詳細
• 細胞内の位置: Host cytoplasm (By similarity). Host cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side (By similarity). Virion (By similarity): E5RPG0 E5RPG0 E5RPG0
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 94336.23

構造登録者

Yoder, J.D.,Hafenstein, S. (登録日: 2012-08-16, 公開日: 2013-05-15, 最終更新日: 2024-02-28)

主引用文献

Cifuente, J.O.,Lee, H.,Yoder, J.D.,Shingler, K.L.,Carnegie, M.S.,Yoder, J.L.,Ashley, R.E.,Makhov, A.M.,Conway, J.F.,Hafenstein, S.
Structures of the procapsid and mature virion of enterovirus 71 strain 1095.
J.Virol., 87:7637-7645, 2013

PubMed Abstract: Enterovirus 71 (EV71) is an important emerging human pathogen with a global distribution and presents a disease pattern resembling poliomyelitis with seasonal epidemics that include cases of severe neurological complications, such as acute flaccid paralysis. EV71 is a member of the Picornaviridae family, which consists of icosahedral, nonenveloped, single-stranded RNA viruses. Here we report structures derived from X-ray crystallography and cryoelectron microscopy (cryo-EM) for the 1095 strain of EV71, including a putative precursor in virus assembly, the procapsid, and the mature virus capsid. The cryo-EM map of the procapsid provides new structural information on portions of the capsid proteins VP0 and VP1 that are disordered in the higher-resolution crystal structures. Our structures solved from virus particles in solution are largely in agreement with those from prior X-ray crystallographic studies; however, we observe small but significant structural differences for the 1095 procapsid compared to a structure solved in a previous study (X. Wang, W. Peng, J. Ren, Z. Hu, J. Xu, Z. Lou, X. Li, W. Yin, X. Shen, C. Porta, T. S. Walter, G. Evans, D. Axford, R. Owen, D. J. Rowlands, J. Wang, D. I. Stuart, E. E. Fry, and Z. Rao, Nat. Struct. Mol. Biol. 19:424-429, 2012) for a different strain of EV71. For both EV71 strains, the procapsid is significantly larger in diameter than the mature capsid, unlike in any other picornavirus. Nonetheless, our results demonstrate that picornavirus capsid expansion is possible without RNA encapsidation and that picornavirus assembly may involve an inward radial collapse of the procapsid to yield the native virion.

PubMed: 23637404
DOI: 10.1128/JVI.03519-12

実験手法

X-RAY DIFFRACTION (3.9 Å)