4GFA

N-terminal coiled-coil dimer of C.elegans SAS-6, crystal form A

4GFA の概要

• エントリーDOI: 10.2210/pdb4gfa/pdb
• 関連するPDBエントリー: 2Y3V 2Y3W 3PYI 3Q0X 3Q0Y 4G79 4GEU 4GEX 4GFC
• 分子名称: Spindle assembly abnormal protein 6, (4S)-2-METHYL-2,4-PENTANEDIOL (2 entities in total)
• 機能のキーワード: beta-sandwich, alpha-beta protein, structural protein, cytoplasmic, centriolar, central tube
• 由来する生物種: Caenorhabditis elegans
• 細胞内の位置: Cytoplasm: O62479
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 83814.35

構造登録者

Erat, M.C.,Vakonakis, I. (登録日: 2012-08-03, 公開日: 2013-06-19, 最終更新日: 2024-02-28)

主引用文献

Hilbert, M.,Erat, M.C.,Hachet, V.,Guichard, P.,Blank, I.D.,Fluckiger, I.,Slater, L.,Lowe, E.D.,Hatzopoulos, G.N.,Steinmetz, M.O.,Gonczy, P.,Vakonakis, I.
Caenorhabditis elegans centriolar protein SAS-6 forms a spiral that is consistent with imparting a ninefold symmetry.
Proc.Natl.Acad.Sci.USA, 110:11373-11378, 2013

PubMed Abstract: Centrioles are evolutionary conserved organelles that give rise to cilia and flagella as well as centrosomes. Centrioles display a characteristic ninefold symmetry imposed by the spindle assembly abnormal protein 6 (SAS-6) family. SAS-6 from Chlamydomonas reinhardtii and Danio rerio was shown to form ninefold symmetric, ring-shaped oligomers in vitro that were similar to the cartwheels observed in vivo during early steps of centriole assembly in most species. Here, we report crystallographic and EM analyses showing that, instead, Caenorhabotis elegans SAS-6 self-assembles into a spiral arrangement. Remarkably, we find that this spiral arrangement is also consistent with ninefold symmetry, suggesting that two distinct SAS-6 oligomerization architectures can direct the same output symmetry. Sequence analysis suggests that SAS-6 spirals are restricted to specific nematodes. This oligomeric arrangement may provide a structural basis for the presence of a central tube instead of a cartwheel during centriole assembly in these species.

PubMed: 23798409
DOI: 10.1073/pnas.1302721110

実験手法

X-RAY DIFFRACTION (3.55 Å)