4GEN

Crystal structure of Zucchini (monomer)

4GEN の概要

• エントリーDOI: 10.2210/pdb4gen/pdb
• 関連するPDBエントリー: 4GEL 4GEM
• 分子名称: Mitochondrial cardiolipin hydrolase, CHLORIDE ION (3 entities in total)
• 機能のキーワード: pirna, phospholipase d, nuclease, hydrolase
• 由来する生物種: Drosophila melanogaster (Fruit fly)
• 細胞内の位置: Mitochondrion outer membrane ; Single-pass membrane protein : Q9VKD7
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 19384.07

構造登録者

Nishimasu, H.,Fukuhara, S.,Ishitani, R.,Nureki, O. (登録日: 2012-08-02, 公開日: 2012-10-17, 最終更新日: 2017-11-15)

主引用文献

Nishimasu, H.,Ishizu, H.,Saito, K.,Fukuhara, S.,Kamatani, M.K.,Bonnefond, L.,Matsumoto, N.,Nishizawa, T.,Nakanaga, K.,Aoki, J.,Ishitani, R.,Siomi, H.,Siomi, M.C.,Nureki, O.
Structure and function of Zucchini endoribonuclease in piRNA biogenesis
Nature, 491:284-287, 2012

PubMed Abstract: PIWI-interacting RNAs (piRNAs) silence transposons to maintain genome integrity in animal germ lines. piRNAs are classified as primary and secondary piRNAs, depending on their biogenesis machinery. Primary piRNAs are processed from long non-coding RNA precursors transcribed from piRNA clusters in the genome through the primary processing pathway. Although the existence of a ribonuclease participating in this pathway has been predicted, its molecular identity remained unknown. Here we show that Zucchini (Zuc), a mitochondrial phospholipase D (PLD) superfamily member, is an endoribonuclease essential for primary piRNA biogenesis. We solved the crystal structure of Drosophila melanogaster Zuc (DmZuc) at 1.75 Å resolution. The structure revealed that DmZuc has a positively charged, narrow catalytic groove at the dimer interface, which could accommodate a single-stranded, but not a double-stranded, RNA. DmZuc and the mouse homologue MmZuc (also known as Pld6 and MitoPLD) showed endoribonuclease activity for single-stranded RNAs in vitro. The RNA cleavage products bear a 5'-monophosphate group, a hallmark of mature piRNAs. Mutational analyses revealed that the conserved active-site residues of DmZuc are critical for the ribonuclease activity in vitro, and for piRNA maturation and transposon silencing in vivo. We propose a model for piRNA biogenesis in animal germ lines, in which the Zuc endoribonuclease has a key role in primary piRNA maturation.

PubMed: 23064230
DOI: 10.1038/nature11509

実験手法

X-RAY DIFFRACTION (2.2 Å)