4GDJ

A subtype N10 neuraminidase-like protein of A/little yellow-shouldered bat/Guatemala/060/2010

4GDJ の概要

• エントリーDOI: 10.2210/pdb4gdj/pdb
• 関連するPDBエントリー: 4GDI
• 分子名称: Neuraminidase, beta-L-fucopyranose-(1-3)-[2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)][alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose, ... (5 entities in total)
• 機能のキーワード: influenza virus, neuraminidase-like, n10, beta propeller, ectodomain, viral protein
• 由来する生物種: Influenza A virus
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 171294.30

構造登録者

Zhu, X.,Wilson, I.A. (登録日: 2012-07-31, 公開日: 2012-09-26, 最終更新日: 2020-07-29)

主引用文献

Zhu, X.,Yang, H.,Guo, Z.,Yu, W.,Carney, P.J.,Li, Y.,Chen, L.M.,Paulson, J.C.,Donis, R.O.,Tong, S.,Stevens, J.,Wilson, I.A.
Crystal structures of two subtype N10 neuraminidase-like proteins from bat influenza A viruses reveal a diverged putative active site.
Proc.Natl.Acad.Sci.USA, 109:18903-18908, 2012

PubMed Abstract: Recently, we reported a unique influenza A virus subtype H17N10 from little yellow-shouldered bats. Its neuraminidase (NA) gene encodes a protein that appears to be highly divergent from all known influenza NAs and was assigned as a new subtype N10. To provide structural and functional insights on the bat H17N10 virus, X-ray structures were determined for N10 NA proteins from influenza A viruses A/little yellow-shouldered bat/Guatemala/164/2009 (GU09-164) in two crystal forms at 1.95 Å and 2.5 Å resolution and A/little yellow-shouldered bat/Guatemala/060/2010 (GU10-060) at 2.0 Å. The overall N10 structures are similar to each other and to other known influenza NA structures, with a single highly conserved calcium binding site in each monomer. However, the region corresponding to the highly conserved active site of influenza A N1-N9 NA subtypes and influenza B NA differs substantially. In particular, most of the amino acid residues required for NA activity are substituted, and the putative active site is much wider because of displacement of the 150-loop and 430-loop. These structural features and the fact that the recombinant N10 protein exhibits no, or extremely low, NA activity suggest that it may have a different function than the NA proteins of other influenza viruses. Accordingly, we propose that the N10 protein be termed an NA-like protein until its function is elucidated.

PubMed: 23012478
DOI: 10.1073/pnas.1212579109

実験手法

X-RAY DIFFRACTION (2 Å)