4GDB

SHV-1 in complex with 4H-pyrazolo[1,5-c][1,3]thiazole containing penem inhibitor

4GDB の概要

• エントリーDOI: 10.2210/pdb4gdb/pdb
• 関連するPDBエントリー: 1Q2P 4GD6 4GD8
• 分子名称: Beta-lactamase SHV-1, (7R)-6-formyl-7-(4H-pyrazolo[1,5-c][1,3]thiazol-2-yl)-4,7-dihydro-1,4-thiazepine-3-carboxylic acid, CYCLOHEXYL-HEXYL-BETA-D-MALTOSIDE, ... (5 entities in total)
• 機能のキーワード: class a beta-lactamase, hydrolase, hydrolase-hydrolase inhibitor complex, hydrolase/hydrolase inhibitor
• 由来する生物種: Klebsiella pneumoniae
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 32824.86

構造登録者

Ke, W.,van den Akker, F. (登録日: 2012-07-31, 公開日: 2013-07-31, 最終更新日: 2024-11-06)

主引用文献

Ke, W.,Pattanaik, P.,Bethel, C.R.,Sheri, A.,Buynak, J.D.,Bonomo, R.A.,van den Akker, F.
Structures of SHV-1 beta-lactamase with penem and penam sulfone inhibitors that form cyclic intermediates stabilized by carbonyl conjugation
Plos One, 7:e49035-e49035, 2012

PubMed Abstract: Bacterial β-lactamase enzymes are in large part responsible for the decreased ability of β-lactam antibiotics to combat infections. The inability to overcome β-lactamase mediated resistance spurred the development of inhibitors with penems and penam sulfones being amongst the most potent and broad spectrum mechanism-based inactivators. These inhibitors form covalent, "suicide-type" inhibitory intermediates that are attached to the catalytic S70 residue. To further probe the details of the mechanism of β-lactamase inhibition by these novel compounds, we determined the crystal structures of SHV-1 bound with penem 1, and penam sulfones SA1-204 and SA3-53. Comparison with each other and with previously determined crystal structures of members of these classes of inhibitors suggests that the final conformation of the covalent adduct can vary greatly amongst the complex structures. In contrast, a common theme of carbonyl conjugation as a mechanism to avoid deacylation emerges despite that the penem and penam sulfone inhibitors form different types of intermediates. The detailed insights gained from this study could be used to further improve new mechanism-based inhibitors of these common class A serine β-lactamases.

PubMed: 23145056
DOI: 10.1371/journal.pone.0049035

実験手法

X-RAY DIFFRACTION (1.84 Å)