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4GCL

structure of no-dna factor

Summary for 4GCL
Entry DOI10.2210/pdb4gcl/pdb
Related4GCK 4GCT 4GCU 4GFK 4GFL
DescriptorNucleoid occlusion factor SlmA, DNA (5'-D(*AP*GP*TP*GP*AP*GP*TP*AP*CP*TP*CP*AP*CP*T)-3'), 2-(N-MORPHOLINO)-ETHANESULFONIC ACID, ... (4 entities in total)
Functional Keywordsdna binding protein, dna binding protein-dna complex, dna binding protein/dna
Biological sourceEscherichia coli
Cellular locationCytoplasm, nucleoid (By similarity): Q1R4V1
Total number of polymer chains12
Total formula weight212469.34
Authors
Schumacher, M.A. (deposition date: 2012-07-30, release date: 2013-06-19, Last modification date: 2024-02-28)
Primary citationTonthat, N.K.,Milam, S.L.,Chinnam, N.,Whitfill, T.,Margolin, W.,Schumacher, M.A.
SlmA forms a higher-order structure on DNA that inhibits cytokinetic Z-ring formation over the nucleoid.
Proc.Natl.Acad.Sci.USA, 110:10586-10591, 2013
Cited by
PubMed Abstract: The spatial and temporal control of Filamenting temperature sensitive mutant Z (FtsZ) Z-ring formation is crucial for proper cell division in bacteria. In Escherichia coli, the synthetic lethal with a defective Min system (SlmA) protein helps mediate nucleoid occlusion, which prevents chromosome fragmentation by binding FtsZ and inhibiting Z-ring formation over the nucleoid. However, to perform its function, SlmA must be bound to the nucleoid. To deduce the basis for this chromosomal requirement, we performed biochemical, cellular, and structural studies. Strikingly, structures show that SlmA dramatically distorts DNA, allowing it to bind as an orientated dimer-of-dimers. Biochemical data indicate that SlmA dimer-of-dimers can spread along the DNA. Combined structural and biochemical data suggest that this DNA-activated SlmA oligomerization would prevent FtsZ protofilament propagation and bundling. Bioinformatic analyses localize SlmA DNA sites near membrane-tethered chromosomal regions, and cellular studies show that SlmA inhibits FtsZ reservoirs from forming membrane-tethered Z rings. Thus, our combined data indicate that SlmA DNA helps block Z-ring formation over chromosomal DNA by forming higher-order protein-nucleic acid complexes that disable FtsZ filaments from coalescing into proper structures needed for Z-ring creation.
PubMed: 23754405
DOI: 10.1073/pnas.1221036110
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (2.65 Å)
Structure validation

239149

数据于2025-07-23公开中

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