4GBK

Crystal structure of aspart insulin at pH 8.5

Summary for 4GBK

• Entry DOI: 10.2210/pdb4gbk/pdb
• Related: 4GBC 4GBI 4GBL 4GBN
• Descriptor: Insulin A chain, Insulin B chain, M-CRESOL, ... (5 entities in total)
• Functional Keywords: t3r3, hormone
• Biological source: Homo sapiens (human); More
• Total number of polymer chains: 4
• Total formula weight: 12089.25

Authors

Lima, L.M.T.R.,Favero-Retto, M.P.,Palmieri, L.C. (deposition date: 2012-07-27, release date: 2013-06-12, Last modification date: 2024-11-06)

Primary citation

Palmieri, L.C.,Favero-Retto, M.P.,Lourenco, D.,Lima, L.M.
A T3R3 hexamer of the human insulin variant B28Asp.
Biophys.Chem., 173:1-7, 2013

PubMed Abstract: Insulin shows a complex equilibrium between monomers and hexamers, involving varying conformers and association states. We sought to perform a structural characterization of the fast-acting human insulin variant B28Asp ("aspart"). Small-angle X-ray scattering measurements reveal similar globular behavior in both the aspart and regular human insulin, with a Rg of 19Å and a Dmax of approximately 50Å, indicating similar mean quaternary assembly distribution. Crystallographic assays revealed a T3R3 assembly of the aspart insulin formed by the TR dimer in the asymmetric unit, with all the first 8 residues of the B chain in the R-state monomer in helical conformation and the participation of its B3Asn in the stabilization of the hexamer. Our data provide access to novel structural information on aspart insulin such as an aspart insulin dimer in solution, the aspart insulin in T conformation and a pure R-state conformer establishing a T3R3 assembly, providing further insight on the stepwise conformational transition and assembly of this fast-insulin.

PubMed: 23428413
DOI: 10.1016/j.bpc.2013.01.003

Experimental method

X-RAY DIFFRACTION (2.4 Å)