4GAL

CRYSTAL STRUCTURE OF HUMAN GALECTIN-7 IN COMPLEX WITH LACTOSE

4GAL の概要

• エントリーDOI: 10.2210/pdb4gal/pdb
• 関連するBIRD辞書のPRD_ID: PRD_900004
• 分子名称: GALECTIN-7, beta-D-galactopyranose-(1-4)-beta-D-glucopyranose (3 entities in total)
• 機能のキーワード: galaptin, lectin, galectin, carbohydrate binding
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 30274.00

構造登録者

Leonidas, D.D.,Acharya, K.R. (登録日: 1998-07-13, 公開日: 1998-11-04, 最終更新日: 2024-04-03)

主引用文献

Leonidas, D.D.,Vatzaki, E.H.,Vorum, H.,Celis, J.E.,Madsen, P.,Acharya, K.R.
Structural basis for the recognition of carbohydrates by human galectin-7.
Biochemistry, 37:13930-13940, 1998

PubMed Abstract: Knowledge about carbohydrate recognition domains of galectins, formerly known as S-type animal lectins, is important in understanding their role(s) in cell-cell interactions. Here we report the crystal structure of human galectin-7 (hGal-7), in free form and in the presence of galactose, galactosamine, lactose, and N-acetyl-lactosamine at high resolution. This is the first structure of a galectin determined in both free and carbohydrate-bound forms. The structure shows a fold similar to that of the prototype galectins -1 and -2, but has greater similarity to a related galectin molecule, Gal-10. Even though the carbohydrate-binding residues are conserved, there are significant changes in this pocket due to shortening of a loop structure. The monomeric hGal-7 molecule exists as a dimer in the crystals, but adopts a packing arrangement considerably different from that of Gal-1 and Gal-2, which has implications for carbohydrate recognition.

PubMed: 9760227
DOI: 10.1021/bi981056x

実験手法

X-RAY DIFFRACTION (1.95 Å)