4G92

CCAAT-binding complex from Aspergillus nidulans with DNA

4G92 の概要

• エントリーDOI: 10.2210/pdb4g92/pdb
• 関連するPDBエントリー: 1N1J 4G91
• 分子名称: HAPB protein, Transcription factor HapC (Eurofung), HapE, ... (7 entities in total)
• 機能のキーワード: transcription factor, nucleosome, minor groove binding, ccaat-binding complex, histone fold motif, specific binding to the ccaat-box, dna, nucleus, transcription-dna complex, transcription/dna
• 由来する生物種: Emericella nidulans; 詳細
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 47525.04

構造登録者

Huber, E.M.,Scharf, D.H.,Hortschansky, P.,Groll, M.,Brakhage, A.A. (登録日: 2012-07-23, 公開日: 2012-10-31, 最終更新日: 2023-09-13)

主引用文献

Huber, E.M.,Scharf, D.H.,Hortschansky, P.,Groll, M.,Brakhage, A.A.
DNA Minor Groove Sensing and Widening by the CCAAT-Binding Complex.
Structure, 20:1757-1768, 2012

PubMed Abstract: The CCAAT box is a frequent element of eukaryotic promoters, and its specific recognition by the conserved heterotrimeric CCAAT-binding complex (CBC) constitutes a key step in promoter organization and regulation of transcription. Here, we report the crystal structures of the CBC from Aspergillus nidulans in the absence and in complex with double-stranded DNA at 1.8 Å resolution. The histone-like subunits HapC and HapE induce nucleosome-like DNA bending by interacting with the sugar-phosphate backbone. Minor groove sensing and widening by subunit HapB tightly anchor the CBC to the CCAAT box, as shown by structural and biochemical data. Furthermore, crucial interactions of the DNA duplex with subunit HapB provide an explanation for the sequence specificity of the CBC. The herein-described mode of transcription factor binding answers the question of how histone proteins gained sequence specificity for the CCAAT box.

PubMed: 22902862
DOI: 10.1016/j.str.2012.07.012

実験手法

X-RAY DIFFRACTION (1.8 Å)