4G68

Biochemical and structural insights into xylan utilization by the thermophilic bacteriumcaldanaerobius polysaccharolyticus

4G68 の概要

• エントリーDOI: 10.2210/pdb4g68/pdb
• 分子名称: ABC transporter, beta-D-xylopyranose-(1-4)-beta-D-xylopyranose-(1-4)-alpha-D-xylopyranose, ... (5 entities in total)
• 機能のキーワード: transporter, transport protein
• 由来する生物種: Caldanaerobius; 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 146789.81

構造登録者

Agarwal, V.,Nair, S.K. (登録日: 2012-07-18, 公開日: 2012-08-22, 最終更新日: 2024-11-27)

主引用文献

Han, Y.,Agarwal, V.,Dodd, D.,Kim, J.,Bae, B.,Mackie, R.I.,Nair, S.K.,Cann, I.K.
Biochemical and Structural Insights into Xylan Utilization by the Thermophilic Bacterium Caldanaerobius polysaccharolyticus.
J.Biol.Chem., 287:34946-34960, 2012

PubMed Abstract: Hemicellulose is the next most abundant plant cell wall component after cellulose. The abundance of hemicellulose such as xylan suggests that their hydrolysis and conversion to biofuels can improve the economics of bioenergy production. In an effort to understand xylan hydrolysis at high temperatures, we sequenced the genome of the thermophilic bacterium Caldanaerobius polysaccharolyticus. Analysis of the partial genome sequence revealed a gene cluster that contained both hydrolytic enzymes and also enzymes key to the pentose-phosphate pathway. The hydrolytic enzymes in the gene cluster were demonstrated to convert products from a large endoxylanase (Xyn10A) predicted to anchor to the surface of the bacterium. We further use structural and calorimetric studies to demonstrate that the end products of Xyn10A hydrolysis of xylan are recognized and bound by XBP1, a putative solute-binding protein, likely for transport into the cell. The XBP1 protein showed preference for xylo-oligosaccharides as follows: xylotriose > xylobiose > xylotetraose. To elucidate the structural basis for the oligosaccharide preference, we solved the co-crystal structure of XBP1 complexed with xylotriose to a 1.8-Å resolution. Analysis of the biochemical data in the context of the co-crystal structure reveals the molecular underpinnings of oligosaccharide length specificity.

PubMed: 22918832
DOI: 10.1074/jbc.M112.391532

実験手法

X-RAY DIFFRACTION (1.8 Å)