4G61

Crystal structure of IMPase/NADP phosphatase complexed with Mg2+ and phosphate

4G61 の概要

• エントリーDOI: 10.2210/pdb4g61/pdb
• 関連するPDBエントリー: 3QMF 3RYD 4G60 4G62 4G64
• 分子名称: Inositol monophosphatase family protein, MAGNESIUM ION, PHOSPHATE ION, ... (9 entities in total)
• 機能のキーワード: impase, nadp phosphatase, fig superfamily, hydrolase
• 由来する生物種: Staphylococcus aureus
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 63897.68

構造登録者

Bhattacharyya, S.,Dutta, D.,Ghosh, A.K.,Das, A.K. (登録日: 2012-07-18, 公開日: 2013-07-24, 最終更新日: 2024-05-29)

主引用文献

Dutta, A.,Bhattacharyya, S.,Dutta, D.,Das, A.K.
Structural elucidation of the binding site and mode of inhibition of Li(+) and Mg(2+) in inositol monophosphatase.
Febs J., 281:5309-5324, 2014

PubMed Abstract: Mg(2+) -dependent, Li(+) -sensitive phosphatases are a widely distributed family of enzymes with significant importance throughout the biological kingdom. Inositol monophosphatase (IMPase) is an important target of Li(+) -based therapeutic agents in manic depressive disorders. However, despite decades of intense research efforts, the precise mechanism of Li(+) -induced inhibition of IMPase remains obscured. Here we describe a structural investigation of the Li(+) binding site in staphylococcal IMPase I (SaIMPase I) using X-ray crystallography. The biochemical study indicated common or overlapping binding sites for Mg(2+) and Li(+) in the active site of SaIMPase I. The crystal structure of the SaIMPase I ternary product complex shows the presence of a phosphate and three Mg(2+) ions (namely Mg1, Mg2 and Mg3) in the active site. As Li(+) is virtually invisible in X-ray crystallography, competitive displacement of Mg(2+) ions from the SaIMPase I ternary product complex as a function of increasing LiCl concentration was used to identify the Li(+) binding site. In this approach, the disappearing electron density of Mg(2+) ions due to Li(+) ion binding was traced, and the Mg(2+) ion present at the Mg2 binding site was found to be replaced. Moreover, based on a detailed comparative investigation of the phosphate orientation and coordination states of Mg(2+) binding sites in enzyme-substrate and enzyme-product complexes, inhibition mechanisms for Li(+) and Mg(2+) are proposed.

PubMed: 25263816
DOI: 10.1111/febs.13070

実験手法

X-RAY DIFFRACTION (2.3 Å)