4G22

Structure of a Lys-HCT mutant from Coffea canephora (Crystal form 1)

4G22 の概要

• エントリーDOI: 10.2210/pdb4g22/pdb
• 関連するPDBエントリー: 4G0B 4G2M
• 分子名称: Hydroxycinnamoyl-CoA shikimate/quinate hydroxycinnamoyltransferase, GLYCEROL, CHLORIDE ION, ... (4 entities in total)
• 機能のキーワード: bahd superfamily, hydroxycinnamoyltransferase, transferase
• 由来する生物種: Coffea canephora (robusta coffee)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 97213.14

構造登録者

McCarthy, A.A.,Lallemand, L.A.,McCarthy, J.G. (登録日: 2012-07-11, 公開日: 2012-08-01, 最終更新日: 2024-11-27)

主引用文献

Lallemand, L.A.,Zubieta, C.,Lee, S.G.,Wang, Y.,Acajjaoui, S.,Timmins, J.,McSweeney, S.,Jez, J.M.,McCarthy, J.G.,McCarthy, A.A.
A structural basis for the biosynthesis of the major chlorogenic acids found in coffee.
Plant Physiol., 160:249-260, 2012

PubMed Abstract: Chlorogenic acids (CGAs) are a group of phenolic secondary metabolites produced by certain plant species and an important component of coffee (Coffea spp.). The CGAs have been implicated in biotic and abiotic stress responses, while the related shikimate esters are key intermediates for lignin biosynthesis. Here, two hydroxycinnamoyl-coenzyme A shikimate/quinate hydroxycinnamoyl transferases (HCT/HQT) from coffee were biochemically characterized. We show, to our knowledge for the first time, that in vitro, HCT is capable of synthesizing the 3,5-O-dicaffeoylquinic acid diester, a major constituent of the immature coffee grain. In order to further understand the substrate specificity and catalytic mechanism of the HCT/HQT, we performed structural and mutagenesis studies of HCT. The three-dimensional structure of a native HCT and a proteolytically stable lysine mutant enabled the identification of important residues involved in substrate specificity and catalysis. Site-directed mutagenesis confirmed the role of residues leucine-400 and phenylalanine-402 in substrate specificity and of histidine-153 and the valine-31 to proline-37 loop in catalysis. In addition, the histidine-154-asparagine mutant was observed to produce 4-fold more dichlorogenic acids compared with the native protein. These data provide, to our knowledge, the first structural characterization of a HCT and, in conjunction with the biochemical and mutagenesis studies presented here, delineate the underlying molecular-level determinants for substrate specificity and catalysis. This work has potential applications in fine-tuning the levels of shikimate and quinate esters (CGAs including dichlorogenic acids) in different plant species in order to generate reduced or elevated levels of the desired target compounds.

PubMed: 22822210
DOI: 10.1104/pp.112.202051

実験手法

X-RAY DIFFRACTION (1.7 Å)