4G1O

Crystal structure of Newcastle disease virus matrix protein

Summary for 4G1O

• Entry DOI: 10.2210/pdb4g1o/pdb
• Related: 4G1G 4G1L
• Descriptor: Matrix protein (2 entities in total)
• Functional Keywords: beta-strand, virus assembly, membrane, virus, viral protein
• Biological source: Newcastle disease virus (NDV)
• Cellular location: Virion (Potential): P11206
• Total number of polymer chains: 2
• Total formula weight: 79598.21

Authors

Meng, G.,Rossmann, M.G. (deposition date: 2012-07-11, release date: 2012-08-22, Last modification date: 2024-02-28)

Primary citation

Battisti, A.J.,Meng, G.,Winkler, D.C.,McGinnes, L.W.,Plevka, P.,Steven, A.C.,Morrison, T.G.,Rossmann, M.G.
Structure and assembly of a paramyxovirus matrix protein.
Proc.Natl.Acad.Sci.USA, 109:13996-14000, 2012

PubMed Abstract: Many pleomorphic, lipid-enveloped viruses encode matrix proteins that direct their assembly and budding, but the mechanism of this process is unclear. We have combined X-ray crystallography and cryoelectron tomography to show that the matrix protein of Newcastle disease virus, a paramyxovirus and relative of measles virus, forms dimers that assemble into pseudotetrameric arrays that generate the membrane curvature necessary for virus budding. We show that the glycoproteins are anchored in the gaps between the matrix proteins and that the helical nucleocapsids are associated in register with the matrix arrays. About 90% of virions lack matrix arrays, suggesting that, in agreement with previous biological observations, the matrix protein needs to dissociate from the viral membrane during maturation, as is required for fusion and release of the nucleocapsid into the host's cytoplasm. Structure and sequence conservation imply that other paramyxovirus matrix proteins function similarly.

PubMed: 22891297
DOI: 10.1073/pnas.1210275109

Experimental method

X-RAY DIFFRACTION (2.2 Å)