4G1G

Crystal structure of Newcastle disease virus matrix protein

4G1G の概要

• エントリーDOI: 10.2210/pdb4g1g/pdb
• 関連するPDBエントリー: 4G1L 4G1O
• 分子名称: Matrix protein (2 entities in total)
• 機能のキーワード: beta strand, virus assembly, membrane, virus, viral protein
• 由来する生物種: Newcastle disease virus (NDV)
• 細胞内の位置: Virion (Potential): P11206
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 79598.21

構造登録者

Meng, G.,Rossmann, M.G. (登録日: 2012-07-10, 公開日: 2012-08-15, 最終更新日: 2024-02-28)

主引用文献

Battisti, A.J.,Meng, G.,Winkler, D.C.,McGinnes, L.W.,Plevka, P.,Steven, A.C.,Morrison, T.G.,Rossmann, M.G.
Structure and assembly of a paramyxovirus matrix protein.
Proc.Natl.Acad.Sci.USA, 109:13996-14000, 2012

PubMed Abstract: Many pleomorphic, lipid-enveloped viruses encode matrix proteins that direct their assembly and budding, but the mechanism of this process is unclear. We have combined X-ray crystallography and cryoelectron tomography to show that the matrix protein of Newcastle disease virus, a paramyxovirus and relative of measles virus, forms dimers that assemble into pseudotetrameric arrays that generate the membrane curvature necessary for virus budding. We show that the glycoproteins are anchored in the gaps between the matrix proteins and that the helical nucleocapsids are associated in register with the matrix arrays. About 90% of virions lack matrix arrays, suggesting that, in agreement with previous biological observations, the matrix protein needs to dissociate from the viral membrane during maturation, as is required for fusion and release of the nucleocapsid into the host's cytoplasm. Structure and sequence conservation imply that other paramyxovirus matrix proteins function similarly.

PubMed: 22891297
DOI: 10.1073/pnas.1210275109

実験手法

X-RAY DIFFRACTION (2.2 Å)