4G0P

Crystal Structure of Arabidopsis thaliana AGO1 MID domain in complex with UMP

4G0P の概要

• エントリーDOI: 10.2210/pdb4g0p/pdb
• 関連するPDBエントリー: 4G0M 4G0O 4G0Q 4G0X 4G0Y 4G0Z
• 分子名称: Protein argonaute 1, URIDINE-5'-MONOPHOSPHATE (3 entities in total)
• 機能のキーワード: mid domain, small rna 5' nucleotide recognition, gene regulation
• 由来する生物種: Arabidopsis thaliana (mouse-ear cress,thale-cress)
• 細胞内の位置: Cytoplasm (Potential): O04379
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 16763.34

構造登録者

Frank, F.,Hauver, J.,Sonenberg, N.,Nagar, B. (登録日: 2012-07-09, 公開日: 2012-07-25, 最終更新日: 2024-02-28)

主引用文献

Frank, F.,Hauver, J.,Sonenberg, N.,Nagar, B.
Arabidopsis Argonaute MID domains use their nucleotide specificity loop to sort small RNAs.
Embo J., 31:3588-3595, 2012

PubMed Abstract: The 5'-nucleotide of small RNAs associates directly with the MID domain of Argonaute (AGO) proteins. In humans, the identity of the 5'-base is sensed by the MID domain nucleotide specificity loop and regulates the integrity of miRNAs. In Arabidopsis thaliana, the 5'-nucleotide also controls sorting of small RNAs into the appropriate member of the AGO family; however, the structural basis for this mechanism is unknown. Here, we present crystal structures of the MID domain from three Arabidopsis AGOs, AtAGO1, AtAGO2 and AtAGO5, and characterize their interactions with nucleoside monophosphates (NMPs). In AtAGOs, the nucleotide specificity loop also senses the identity of the 5'-nucleotide but uses more diverse modes of recognition owing to the greater complexity of small RNAs found in plants. Binding analyses of these interactions reveal a strong correlation between their affinities and evolutionary conservation.

PubMed: 22850669
DOI: 10.1038/emboj.2012.204

実験手法

X-RAY DIFFRACTION (1.8 Å)