4FQG

Crystal structure of the TCERG1 FF4-6 tandem repeat domain

4FQG の概要

• エントリーDOI: 10.2210/pdb4fqg/pdb
• 分子名称: Transcription elongation regulator 1, NICKEL (II) ION, CHLORIDE ION, ... (4 entities in total)
• 機能のキーワード: ff domain, tandem repeat domain, pctd-binding domain, transcription
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 46327.82

構造登録者

Liu, J.,Fan, S.,Lee, C.J.,Greenleaf, A.L.,Zhou, P. (登録日: 2012-06-25, 公開日: 2013-02-27, 最終更新日: 2024-03-13)

主引用文献

Liu, J.,Fan, S.,Lee, C.J.,Greenleaf, A.L.,Zhou, P.
Specific Interaction of the Transcription Elongation Regulator TCERG1 with RNA Polymerase II Requires Simultaneous Phosphorylation at Ser2, Ser5, and Ser7 within the Carboxyl-terminal Domain Repeat.
J.Biol.Chem., 288:10890-10901, 2013

PubMed Abstract: The human transcription elongation regulator TCERG1 physically couples transcription elongation and splicing events by interacting with splicing factors through its N-terminal WW domains and the hyperphosphorylated C-terminal domain (CTD) of RNA polymerase II through its C-terminal FF domains. Here, we report biochemical and structural characterization of the C-terminal three FF domains (FF4-6) of TCERG1, revealing a rigid integral domain structure of the tandem FF repeat that interacts with the hyperphosphorylated CTD (PCTD). Although FF4 and FF5 adopt a classical FF domain fold containing three orthogonally packed α helices and a 310 helix, FF6 contains an additional insertion helix between α1 and α2. The formation of the integral tandem FF4-6 repeat is achieved by merging the last helix of the preceding FF domain and the first helix of the following FF domain and by direct interactions between neighboring FF domains. Using peptide column binding assays and NMR titrations, we show that binding of the FF4-6 tandem repeat to the PCTD requires simultaneous phosphorylation at Ser(2), Ser(5), and Ser(7) positions within two consecutive Y(1)S(2)P(3)T(4)S(5)P(6)S(7) heptad repeats. Such a sequence-specific PCTD recognition is achieved through CTD-docking sites on FF4 and FF5 of TCERG1 but not FF6. Our study presents the first example of a nuclear factor requiring all three phospho-Ser marks within the heptad repeat of the CTD for high affinity binding and provides a molecular interpretation for the biochemical connection between the Ser(7) phosphorylation enrichment in the CTD of the transcribing RNA polymerase II over introns and co-transcriptional splicing events.

PubMed: 23436654
DOI: 10.1074/jbc.M113.460238

実験手法

X-RAY DIFFRACTION (2 Å)