4FOF

Crystal Structure of the blue-light absorbing form of the Thermosynechococcus elongatus PixJ GAF-domain

4FOF の概要

• エントリーDOI: 10.2210/pdb4fof/pdb
• 分子名称: Methyl-accepting chemotaxis protein, Phycoviolobilin, blue light-absorbing form, TRIETHYLENE GLYCOL, ... (4 entities in total)
• 機能のキーワード: phytochrome, cyanobacteriochrome, phycoviolobilin, photoreceptor, gaf-domain, blue/green photoreceptor, signaling protein
• 由来する生物種: Thermosynechococcus elongatus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 20339.07

構造登録者

Burgie, E.S.,Walker, J.M.,Phillips Jr., G.N.,Vierstra, R.D. (登録日: 2012-06-20, 公開日: 2013-01-16, 最終更新日: 2024-10-16)

主引用文献

Burgie, E.S.,Walker, J.M.,Phillips, G.N.,Vierstra, R.D.
A Photo-Labile Thioether Linkage to Phycoviolobilin Provides the Foundation for the Blue/Green Photocycles in DXCF-Cyanobacteriochromes.
Structure, 21:88-97, 2013

PubMed Abstract: The phytochrome superfamily encompasses a diverse collection of photochromic photoreceptors in plants and microorganisms that employ a covalently linked bilin cradled in a cGMP-phosphodiesterase/adenylyl-cyclase/FhlA (GAF) domain to detect light. Whereas most interconvert between red- and far-red-light-absorbing states, cyanobacteria also express variants called cyanobacteriochromes (CBCRs) that modify bilin absorption to collectively perceive the entire visible spectrum. Here, we present two X-ray crystallographic structures of the GAF domain from the blue/green photochromic CBCR PixJ from Thermosynechococcus elongatus. These structures confirm the hypothesis that CBCRs variably manipulate the chromophore π-conjugation system through isomerization and a second thioether linkage, in this case involving the bilin C10 carbon and Cys494 within a DXCF sequence characteristic of blue/green CBCRs. Biochemical studies support a mechanism for photoconversion whereby the second linkage ruptures on route to the green-light-absorbing state. Collectively, the TePixJ(GAF) models illustrate the remarkable structural and photochemical versatility among phytochromes and CBCRs in driving light perception.

PubMed: 23219880
DOI: 10.1016/j.str.2012.11.001

実験手法

X-RAY DIFFRACTION (2.416 Å)