4FMC

EspG-Rab1 complex

4FMC の概要

• エントリーDOI: 10.2210/pdb4fmc/pdb
• 関連するPDBエントリー: 4FMA 4FMB 4FMD 4FME
• 分子名称: ROrf2, Ras-related protein Rab-1A, TRIETHYLENE GLYCOL, ... (8 entities in total)
• 機能のキーワード: alpha-beta fold, rab1-gap, rab1, protein binding
• 由来する生物種: Escherichia coli; 詳細
• 細胞内の位置: Golgi apparatus: P62820 P62820
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 170794.86

構造登録者

Shao, F.,Zhu, Y. (登録日: 2012-06-16, 公開日: 2012-09-05, 最終更新日: 2024-02-28)

主引用文献

Dong, N.,Zhu, Y.,Lu, Q.,Hu, L.,Zheng, Y.,Shao, F.
Structurally Distinct Bacterial TBC-like GAPs Link Arf GTPase to Rab1 Inactivation to Counteract Host Defenses.
Cell(Cambridge,Mass.), 150:1029-1041, 2012

PubMed Abstract: Rab GTPases are frequent targets of vacuole-living bacterial pathogens for appropriate trafficking of the vacuole. Here we discover that bacterial effectors including VirA from nonvacuole Shigella flexneri and EspG from extracellular Enteropathogenic Escherichia coli (EPEC) harbor TBC-like dual-finger motifs and exhibits potent RabGAP activities. Specific inactivation of Rab1 by VirA/EspG disrupts ER-to-Golgi trafficking. S. flexneri intracellular persistence requires VirA TBC-like GAP activity that mediates bacterial escape from autophagy-mediated host defense. Rab1 inactivation by EspG severely blocks host secretory pathway, resulting in inhibited interleukin-8 secretion from infected cells. Crystal structures of VirA/EspG-Rab1-GDP-aluminum fluoride complexes highlight TBC-like catalytic role for the arginine and glutamine finger residues and reveal a 3D architecture distinct from that of the TBC domain. Structure of Arf6-EspG-Rab1 ternary complex illustrates a pathogenic signaling complex that rewires host Arf signaling to Rab1 inactivation. Structural distinctions of VirA/EspG further predict a possible extensive presence of TBC-like RabGAP effectors in counteracting various host defenses.

PubMed: 22939626
DOI: 10.1016/j.cell.2012.06.050

実験手法

X-RAY DIFFRACTION (2.8 Å)