4FLN

Crystal structure of plant protease Deg2

4FLN の概要

• エントリーDOI: 10.2210/pdb4fln/pdb
• 分子名称: Protease Do-like 2, chloroplastic, Unknown peptide, ... (4 entities in total)
• 機能のキーワード: protease, deg, pdz, hydrolase
• 由来する生物種: Arabidopsis thaliana (mouse-ear cress,thale-cress); 詳細
• 細胞内の位置: Plastid, chloroplast thylakoid membrane; Peripheral membrane protein; Stromal side: O82261
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 181565.11

構造登録者

Gong, W.,Liu, L.,Sun, R.,Gao, F. (登録日: 2012-06-15, 公開日: 2012-09-19, 最終更新日: 2024-02-28)

主引用文献

Sun, R.,Fan, H.,Gao, F.,Lin, Y.,Zhang, L.,Gong, W.,Liu, L.
Crystal structure of Arabidopsis deg2 protein reveals an internal PDZ ligand locking the hexameric resting state.
J.Biol.Chem., 287:37564-37569, 2012

PubMed Abstract: Eukaryotic organelles have developed elaborate protein quality control systems to ensure their normal activity, among which Deg/HtrA proteases play an essential role. Plant Deg2 protease is a homologue of prokaryotic DegQ/DegP proteases and is located in the chloroplast stroma, where its proteolytic activity is required to maintain the efficiency of photosynthetic machinery during stress. Here, we demonstrate that Deg2 exhibits dual protease-chaperone activities, and we present the hexameric structure of Deg2 complexed with co-purified peptides. The structure shows that Deg2 contains a unique second PDZ domain (PDZ2) following a conventional PDZ domain (PDZ1), with PDZ2 orchestrating the cage assembly of Deg2. We discovered a conserved internal ligand for PDZ2 that mediates hexamer formation and thus locks the protease in the resting state. These findings provide insight into the diverse modes of PDZ domain-mediated regulation of Deg proteases.

PubMed: 22961982
DOI: 10.1074/jbc.M112.394585

実験手法

X-RAY DIFFRACTION (2.8 Å)