4FIT

FHIT-APO

4FIT の概要

• エントリーDOI: 10.2210/pdb4fit/pdb
• 分子名称: FRAGILE HISTIDINE TRIAD PROTEIN (2 entities in total)
• 機能のキーワード: hydrolase, fragile histidine triad protein, fhit, putative tumor suppressor, hit protein family, histidine triad protein family, nucleotidyl hydrolase, nucleotidyl transferase
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Cytoplasm: P49789
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 16886.20

構造登録者

Lima, C.D.,Klein, M.G.,Hendrickson, W.A. (登録日: 1997-09-25, 公開日: 1998-03-25, 最終更新日: 2024-02-28)

主引用文献

Lima, C.D.,Klein, M.G.,Hendrickson, W.A.
Structure-based analysis of catalysis and substrate definition in the HIT protein family.
Science, 278:286-290, 1997

PubMed Abstract: The histidine triad (HIT) protein family is among the most ubiquitous and highly conserved in nature, but a biological activity has not yet been identified for any member of the HIT family. Fragile histidine triad protein (FHIT) and protein kinase C interacting protein (PKCI) were used in a structure-based approach to elucidate characteristics of in vivo ligands and reactions. Crystallographic structures of apo, substrate analog, pentacovalent transition-state analog, and product states of both enzymes reveal a catalytic mechanism and define substrate characteristics required for catalysis, thus unifying the HIT family as nucleotidyl hydrolases, transferases, or both. The approach described here may be useful in identifying structure-function relations between protein families identified through genomics.

PubMed: 9323207
DOI: 10.1126/science.278.5336.286

実験手法

X-RAY DIFFRACTION (2.5 Å)