4FHR

Crystal structure of the complex between the flagellar motor proteins FliG and FliM.

4FHR の概要

• エントリーDOI: 10.2210/pdb4fhr/pdb
• 関連するPDBエントリー: 1LKV 3AJC 3HJL
• 分子名称: Flagellar motor switch protein FliM, Flagellar motor switch protein FliG (3 entities in total)
• 機能のキーワード: flagellar motor, motor protein
• 由来する生物種: Thermotoga maritima; 詳細
• 細胞内の位置: Cell membrane; Peripheral membrane protein; Cytoplasmic side (By similarity): Q9WY63
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 46095.15

構造登録者

Paz, A.,Vartanian, A.S.,Fortgang, E.A.,Abramson, J.,Dahlquist, F.W. (登録日: 2012-06-06, 公開日: 2012-08-22, 最終更新日: 2023-09-13)

主引用文献

Vartanian, A.S.,Paz, A.,Fortgang, E.A.,Abramson, J.,Dahlquist, F.W.
Structure of flagellar motor proteins in complex allows for insights into motor structure and switching.
J.Biol.Chem., 287:35779-35783, 2012

PubMed Abstract: The flagellar motor is one type of propulsion device of motile bacteria. The cytoplasmic ring (C-ring) of the motor interacts with the stator to generate torque in clockwise and counterclockwise directions. The C-ring is composed of three proteins, FliM, FliN, and FliG. Together they form the "switch complex" and regulate switching and torque generation. Here we report the crystal structure of the middle domain of FliM in complex with the middle and C-terminal domains of FliG that shows the interaction surface and orientations of the proteins. In the complex, FliG assumes a compact conformation in which the middle and C-terminal domains (FliG(MC)) collapse and stack together similarly to the recently published structure of a mutant of FliG(MC) with a clockwise rotational bias. This intramolecular stacking of the domains is distinct from the intermolecular stacking seen in other structures of FliG. We fit the complex structure into the three-dimensional reconstructions of the motor and propose that the cytoplasmic ring is assembled from 34 FliG and FliM molecules in a 1:1 fashion.

PubMed: 22896702
DOI: 10.1074/jbc.C112.378380

実験手法

X-RAY DIFFRACTION (1.931 Å)