4FH8

Crystal Structure of Peroxiredoxin-1 from the human hookworm Ancylostoma ceylanicum

4FH8 の概要

• エントリーDOI: 10.2210/pdb4fh8/pdb
• 関連するPDBエントリー: 1QMV 3TJB 4FH9
• 分子名称: AcePrx-1 (2 entities in total)
• 機能のキーワード: 2-cys peroxiredoxin, oxidoreductase
• 由来する生物種: Ancylostoma ceylanicum
• タンパク質・核酸の鎖数: 10
• 化学式量合計: 229983.12

構造登録者

Nguyen, J.B.,Modis, Y. (登録日: 2012-06-05, 公開日: 2013-06-12, 最終更新日: 2023-09-13)

主引用文献

Nguyen, J.B.,Pool, C.D.,Wong, C.Y.,Treger, R.S.,Williams, D.L.,Cappello, M.,Lea, W.A.,Simeonov, A.,Vermeire, J.J.,Modis, Y.
Peroxiredoxin-1 from the Human Hookworm Ancylostoma ceylanicum Forms a Stable Oxidized Decamer and Is Covalently Inhibited by Conoidin A.
Chem.Biol., 20:991-1001, 2013

PubMed Abstract: Hookworms are parasitic nematodes that have a devastating impact on global health, particularly in developing countries. We report a biochemical and structural analysis of a peroxiredoxin from the hookworm Ancylostoma ceylanicum, AcePrx-1. Peroxiredoxins provide antioxidant protection and act as signaling molecules and chaperones. AcePrx-1 is expressed in adult hookworms and can be inactivated by 2,3-bis(bromomethyl)quinoxaline-1,4-dioxide (conoidin A). Conoidin A inactivates AcePrx-1 by alkylating or crosslinking the catalytic cysteines, while maintaining the enzyme in the "locally unfolded" conformation. Irreversible oxidation of the resolving cysteine may contribute additional inhibitory activity. A crystal structure of oxidized AcePrx-1 reveals a disulfide-linked decamer. A helix macrodipole near the active site increases the reactivity of the catalytic cysteines to conoidin A. This work demonstrates the promise of conoidin compounds as probes to evaluate peroxiredoxins as drug targets in human parasites.

PubMed: 23891152
DOI: 10.1016/j.chembiol.2013.06.011

実験手法

X-RAY DIFFRACTION (2.11 Å)