4F9P

Crystal Structure of the Human BTN3A1 Ectodomain in Complex with the 103.2 Single Chain Antibody

4F9P の概要

• エントリーDOI: 10.2210/pdb4f9p/pdb
• 分子名称: Butyrophilin subfamily 3 member A1, 103.2 anti-BTN3A1 antibody fragment (2 entities in total)
• 機能のキーワード: b7 superfamily, butyrophilin, cd277, immune system
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Cell membrane; Single-pass type I membrane protein: O00481
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 100613.67

構造登録者

Palakodeti, A.,Sandstrom, A.,Sundaresan, L.,Harly, C.,Nedellec, S.,Olive, D.,Scotet, E.,Bonneville, M.,Adams, E.J. (登録日: 2012-05-19, 公開日: 2012-08-08, 最終更新日: 2024-11-06)

主引用文献

Palakodeti, A.,Sandstrom, A.,Sundaresan, L.,Harly, C.,Nedellec, S.,Olive, D.,Scotet, E.,Bonneville, M.,Adams, E.J.
The molecular basis for modulation of human V(gamma)9V(delta)2 T cell responses by CD277/Butyrophilin-3 (BTN3A)-specific antibodies
J.Biol.Chem., 287:32780-32790, 2012

PubMed Abstract: Human Vγ9Vδ2 T cells are well known for their rapid and potent response to infection and tumorigenesis when in the presence of endogenous or exogenous phosphoisoprenoids. However, the molecular mechanisms behind the activation of this γδ T cell population remains unclear. Evidence pointing to a role for the CD277/butyrophilin-3 (BTN3A) molecules in this response led us to investigate the structures of these molecules and their modifications upon binding to an agonist antibody (20.1) that mimics phosphoisoprenoid-mediated Vγ9Vδ2 activation and an antagonist antibody (103.2) that inhibits this reactivity. We find that the three BTN3A isoforms: BTN3A1, BTN3A2, and BTN3A3, have high structural homology to the B7 superfamily of proteins and exist as V-shaped homodimers in solution, associating through the membrane proximal C-type Ig domain. The 20.1 and 103.2 antibodies bind to separate epitopes on the BTN3A Ig-V domain with high affinity but likely with different valencies based on their binding orientation. These structures directly complement functional studies of this system that demonstrate that BTN3A1 is necessary for Vγ9Vδ2 activation and begin to unravel the extracellular events that occur during stimulation through the Vγ9Vδ2 T cell receptor.

PubMed: 22846996
DOI: 10.1074/jbc.M112.384354

実験手法

X-RAY DIFFRACTION (3.519 Å)