4F9F

Crystal Structure of VldE, the pseudo-glycosyltransferase, in complex with GDP and Trehalose

4F9F の概要

• エントリーDOI: 10.2210/pdb4f9f/pdb
• 関連するPDBエントリー: 3VDM 3VDN 4F96 4F97
• 関連するBIRD辞書のPRD_ID: PRD_900006
• 分子名称: VldE, alpha-D-glucopyranose-(1-1)-alpha-D-glucopyranose, GUANOSINE-5'-DIPHOSPHATE, ... (4 entities in total)
• 機能のキーワード: twin rossmann fold, transferase
• 由来する生物種: Streptomyces hygroscopicus subsp. limoneus
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 333383.01

構造登録者

Cavalier, M.C.,Yim, Y.-S.,Asamizu, S.,Neau, D.,Almabruk, K.H.,Mahmud, T.,Lee, Y.-H. (登録日: 2012-05-18, 公開日: 2012-10-17, 最終更新日: 2024-02-28)

主引用文献

Cavalier, M.C.,Yim, Y.S.,Asamizu, S.,Neau, D.,Almabruk, K.H.,Mahmud, T.,Lee, Y.H.
Mechanistic Insights into Validoxylamine A 7'-Phosphate Synthesis by VldE Using the Structure of the Entire Product Complex.
Plos One, 7:e44934-e44934, 2012

PubMed Abstract: The pseudo-glycosyltransferase VldE catalyzes non-glycosidic C-N coupling between an unsaturated cyclitol and a saturated aminocyclitol with the conservation of the stereochemical configuration of the substrates to form validoxylamine A 7'-phosphate, the biosynthetic precursor of the antibiotic validamycin A. To study the molecular basis of its mechanism, the three-dimensional structures of VldE from Streptomyces hygroscopicus subsp. limoneus was determined in apo form, in complex with GDP, in complex with GDP and validoxylamine A 7'-phosphate, and in complex with GDP and trehalose. The structure of VldE with the catalytic site in both an "open" and "closed" conformation is also described. With these structures, the preferred binding of the guanine moiety by VldE, rather than the uracil moiety as seen in OtsA could be explained. The elucidation of the VldE structure in complex with the entirety of its products provides insight into the internal return mechanism by which catalysis occurs with a net retention of the stereochemical configuration of the donated cyclitol.

PubMed: 23028689
DOI: 10.1371/journal.pone.0044934

実験手法

X-RAY DIFFRACTION (2.807 Å)