4F4B

Structure of OSH4 with a cholesterol analog

Summary for 4F4B

• Entry DOI: 10.2210/pdb4f4b/pdb
• Related: 1zhx 1zhy
• Descriptor: Protein KES1, (3beta,9beta,25R)-3-hydroxy-26-iodocholest-5-ene-16,22-dione (3 entities in total)
• Functional Keywords: oxysterol, sterol binding protein, lipid binding protein
• Biological source: Saccharomyces cerevisiae (Baker's yeast)
• Cellular location: Golgi apparatus membrane : P35844
• Total number of polymer chains: 2
• Total formula weight: 100621.23

Authors

Koag, M.C.,Monzingo, A.F.,Cheun, Y.,Lee, S. (deposition date: 2012-05-10, release date: 2013-05-15, Last modification date: 2024-02-28)

Primary citation

Koag, M.C.,Cheun, Y.,Kou, Y.,Ouzon-Shubeita, H.,Min, K.,Monzingo, A.F.,Lee, S.
Synthesis and structure of 16,22-diketocholesterol bound to oxysterol-binding protein Osh4.
Steroids, 78:938-944, 2013

PubMed Abstract: We have synthesized 16,22-diketocholesterol, a novel ligand for oxysterol-binding protein Osh4, and determined X-ray structure of the diketocholesterol in complex with Osh4. The X-ray structure shows that α7 helix of Osh4 assumes open conformation while the rest of Osh4, closed conformation, implying this diketocholesterol-bound Osh4 structure may represent a structural intermediate between the two conformations.

PubMed: 23756172
DOI: 10.1016/j.steroids.2013.05.016

Experimental method

X-RAY DIFFRACTION (1.87 Å)