4F4B

Structure of OSH4 with a cholesterol analog

4F4B の概要

• エントリーDOI: 10.2210/pdb4f4b/pdb
• 関連するPDBエントリー: 1zhx 1zhy
• 分子名称: Protein KES1, (3beta,9beta,25R)-3-hydroxy-26-iodocholest-5-ene-16,22-dione (3 entities in total)
• 機能のキーワード: oxysterol, sterol binding protein, lipid binding protein
• 由来する生物種: Saccharomyces cerevisiae (Baker's yeast)
• 細胞内の位置: Golgi apparatus membrane : P35844
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 100621.23

構造登録者

Koag, M.C.,Monzingo, A.F.,Cheun, Y.,Lee, S. (登録日: 2012-05-10, 公開日: 2013-05-15, 最終更新日: 2024-02-28)

主引用文献

Koag, M.C.,Cheun, Y.,Kou, Y.,Ouzon-Shubeita, H.,Min, K.,Monzingo, A.F.,Lee, S.
Synthesis and structure of 16,22-diketocholesterol bound to oxysterol-binding protein Osh4.
Steroids, 78:938-944, 2013

PubMed Abstract: We have synthesized 16,22-diketocholesterol, a novel ligand for oxysterol-binding protein Osh4, and determined X-ray structure of the diketocholesterol in complex with Osh4. The X-ray structure shows that α7 helix of Osh4 assumes open conformation while the rest of Osh4, closed conformation, implying this diketocholesterol-bound Osh4 structure may represent a structural intermediate between the two conformations.

PubMed: 23756172
DOI: 10.1016/j.steroids.2013.05.016

実験手法

X-RAY DIFFRACTION (1.87 Å)