4F3T

Human Argonaute-2 - miR-20a complex

4F3T の概要

• エントリーDOI: 10.2210/pdb4f3t/pdb
• 分子名称: Protein argonaute-2, RNA (5'-R(P*UP*AP*AP*AP*GP*UP*GP*CP*UP*UP*AP*UP*AP*GP*UP*G*CP*AP*GP*G)-3'), PHENOL, ... (4 entities in total)
• 機能のキーワード: hydrolase/gene regulation, rnai, slicer, rna, hydrolase-rna complex, hydrolase/rna
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Cytoplasm, P-body : Q9UKV8
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 104115.39

構造登録者

Elkayam, E.,Kuhn, C.-D.,Tocilj, A.,Joshua-Tor, L. (登録日: 2012-05-09, 公開日: 2012-05-30, 最終更新日: 2024-02-28)

主引用文献

Elkayam, E.,Kuhn, C.D.,Tocilj, A.,Haase, A.D.,Greene, E.M.,Hannon, G.J.,Joshua-Tor, L.
The Structure of Human Argonaute-2 in Complex with miR-20a.
Cell(Cambridge,Mass.), 150:100-110, 2012

PubMed Abstract: Argonaute proteins lie at the heart of the RNA-induced silencing complex (RISC), wherein they use small RNA guides to recognize targets. Initial insight into the architecture of Argonautes came from studies of prokaryotic proteins, revealing a crescent-shaped base made up of the amino-terminal, PAZ, middle, and PIWI domains. The recently reported crystal structure of human Argonaute-2 (hAgo2), the "slicer" in RNA interference, in complex with a mixed population of RNAs derived from insect cells provides insight into the architecture of a eukaryotic Argonaute protein with defined biochemical and biological functions. Here, we report the structure of human Ago2 bound to a physiologically relevant microRNA, microRNA-20a, at 2.2 Å resolution. The miRNA is anchored at both ends by the Mid and PAZ domains and makes several kinks and turns along the binding groove. Interestingly, miRNA binding confers remarkable stability on hAgo2, locking this otherwise flexible enzyme into a stable conformation.

PubMed: 22682761
DOI: 10.1016/j.cell.2012.05.017

実験手法

X-RAY DIFFRACTION (2.25 Å)