4ES4

Crystal structure of YdiV and FlhD complex

4ES4 の概要

• エントリーDOI: 10.2210/pdb4es4/pdb
• 関連するPDBエントリー: 3TLQ
• 分子名称: Putative cyclic di-GMP regulator CdgR, Flagellar transcriptional regulator FlhD (2 entities in total)
• 機能のキーワード: flagellar regulation, transcription
• 由来する生物種: Escherichia coli; 詳細
• 細胞内の位置: Cytoplasm: P0A8S9
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 161614.08

構造登録者

Li, B.,Gu, L. (登録日: 2012-04-22, 公開日: 2012-10-10, 最終更新日: 2023-11-08)

主引用文献

Li, B.,Li, N.,Wang, F.,Guo, L.,Huang, Y.,Liu, X.,Wei, T.,Zhu, D.,Liu, C.,Pan, H.,Xu, S.,Wang, H.W.,Gu, L.
Structural insight of a concentration-dependent mechanism by which YdiV inhibits Escherichia coli flagellum biogenesis and motility
Nucleic Acids Res., 40:11073-11085, 2012

PubMed Abstract: YdiV is a negative regulator of cell motility. It interacts with FlhD(4)C(2) complex, a product of flagellar master operon, which works as the transcription activator of all other flagellar operons. Here, we report the crystal structures of YdiV and YdiV(2)-FlhD(2) complex at 1.9 Å and 2.9 Å resolutions, respectively. Interestingly, YdiV formed multiple types of complexes with FlhD(4)C(2). YdiV(1)-FlhD(4)C(2) and YdiV(2)-FlhD(4)C(2) still bound to DNA, while YdiV(3)-FlhD(4)C(2) and YdiV(4)-FlhD(4)C(2) did not. DNA bound FlhD(4)C(2) through wrapping around the FlhC subunit rather than the FlhD subunit. Structural analysis showed that only two peripheral FlhD subunits were accessible for YdiV binding, forming the YdiV(2)-FlhD(4)C(2) complex without affecting the integrity of ring-like structure. YdiV(2)-FlhD(2) structure and the negative staining electron microscopy reconstruction of YdiV(4)-FlhD(4)C(2) suggested that the third and fourth YdiV molecule bound to the FlhD(4)C(2) complex through squeezing into the ring-like structure of FlhD(4)C(2) between the two internal D subunits. Consequently, the ring-like structure opened up, and the complex lost DNA-binding ability. Thus, YdiV inhibits FlhD(4)C(2) only at relatively high concentrations.

PubMed: 23002140
DOI: 10.1093/nar/gks869

実験手法

X-RAY DIFFRACTION (2.9 Å)