4EQ7

Structure of Atu4243-GABA receptor

4EQ7 の概要

• エントリーDOI: 10.2210/pdb4eq7/pdb
• 分子名称: ABC transporter, substrate binding protein (Polyamine), SULFATE ION, GLYCEROL, ... (4 entities in total)
• 機能のキーワード: class d of pbp, transport protein
• 由来する生物種: Agrobacterium tumefaciens
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 72278.26

構造登録者

Morera, S.,Planamente, S. (登録日: 2012-04-18, 公開日: 2012-11-21, 最終更新日: 2024-10-16)

主引用文献

Planamente, S.,Mondy, S.,Hommais, F.,Vigouroux, A.,Morera, S.,Faure, D.
Structural basis for selective GABA binding in bacterial pathogens.
Mol.Microbiol., 86:1085-1099, 2012

PubMed Abstract: GABA acts as an intercellular signal in eukaryotes and as an interspecies signal in host-microbe interactions. Structural characteristics of selective eukaryotic GABA receptors and bacterial GABA sensors are unknown. Here, we identified the selective GABA-binding protein, called Atu4243, in the plant pathogen Agrobacterium tumefaciens. A constructed atu4243 mutant was affected in GABA transport and in expression of the GABA-regulated functions, including aggressiveness on two plant hosts and degradation of the quorum-sensing signal. The GABA-bound Atu4243 structure at 1.28 Å reveals that GABA adopts a conformation never observed so far and interacts with two key residues, Arg(203) and Asp(226) of which the role in GABA binding and GABA signalling in Agrobacterium has been validated using appropriate mutants. The conformational GABA-analogue trans-4-aminocrotonic acid (TACA) antagonizes GABA activity, suggesting structural similarities between the binding sites of the bacterial sensor Atu4243 and mammalian GABA(C) receptors. Exploration of genomic databases reveals Atu4243 orthologues in several pathogenic and symbiotic proteobacteria, such as Rhizobium, Azospirillum, Burkholderia and Pseudomonas. Thus, this study establishes a structural basis for selective GABA sensors and offers opportunities for deciphering the role of the GABA-mediated communication in several host-pathogen interactions.

PubMed: 23043322
DOI: 10.1111/mmi.12043

実験手法

X-RAY DIFFRACTION (1.91 Å)