4EO1

crystal structure of the TolA binding domain from the filamentous phage IKe

4EO1 の概要

• エントリーDOI: 10.2210/pdb4eo1/pdb
• 関連するPDBエントリー: 1fgp 1g3p 1tol 2x9a 2x9b 3dgs 3knq 4EO0
• 分子名称: Attachment protein G3P, MAGNESIUM ION (3 entities in total)
• 機能のキーワード: tola binding protein, infection, g3p, filamentous phage, attachment protein, tola binding, coat protein, tola, phage coat, phage envelope of the filamentous phage ike, viral protein
• 由来する生物種: Enterobacteria phage Ike
• 細胞内の位置: Virion : P03663
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 7512.36

構造登録者

Jakob, R.P.,Geitner, A.J.,Weininger, U.,Balbach, J.,Dobbek, H.,Schmid, F.X. (登録日: 2012-04-13, 公開日: 2012-05-30, 最終更新日: 2024-10-30)

主引用文献

Jakob, R.P.,Geitner, A.J.,Weininger, U.,Balbach, J.,Dobbek, H.,Schmid, F.X.
Structural and energetic basis of infection by the filamentous bacteriophage IKe.
Mol.Microbiol., 84:1124-1138, 2012

PubMed Abstract: Filamentous phage use the two N-terminal domains of their gene-3-proteins to initiate infection of Escherichia coli. One domain interacts with a pilus, and then the other domain binds to TolA at the cell surface. In phage fd, these two domains are tightly associated with each other, which renders the phage robust but non-infectious, because the TolA binding site is inaccessible. Activation for infection requires partial unfolding, domain disassembly and prolyl isomerization. Phage IKe infects E. coli less efficiently than phage fd. Unlike in phage fd, the pilus- and TolA-binding domains of phage IKe are independent of each other in stability and folding. The site for TolA binding is thus always accessible, but the affinity is very low. The structures of the two domains, analysed by X-ray crystallography and by NMR spectroscopy, revealed a unique fold for the N-pilus-binding domain and a conserved fold for the TolA-binding domain. The absence of an activation mechanism as in phage fd and the low affinity for TolA probably explain the low infectivity of phage IKe. They also explain why, in a previous co-evolution experiment with a mixture of phage fd and phage IKe, all hybrid phage adopted the superior infection mechanism of phage fd.

PubMed: 22591114
DOI: 10.1111/j.1365-2958.2012.08079.x

実験手法

X-RAY DIFFRACTION (1.8 Å)