4EHU

Activator of the 2-Hydroxyisocaproyl-CoA Dehydratase from Clostridium difficile with bound ADPNP

4EHU の概要

• エントリーDOI: 10.2210/pdb4ehu/pdb
• 関連するPDBエントリー: 4EHT 4EIA
• 分子名称: Activator of 2-hydroxyisocaproyl-CoA dehydratase, IRON/SULFUR CLUSTER, PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER, ... (7 entities in total)
• 機能のキーワード: actin fold, atpase, electron transfer, atp/adp binding, 2-hydroxyisocaproyl-coa dehydratase binding, electron transport
• 由来する生物種: Clostridium difficile
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 60970.46

構造登録者

Knauer, S.H.,Dobbek, H. (登録日: 2012-04-04, 公開日: 2012-08-08, 最終更新日: 2023-09-13)

主引用文献

Knauer, S.H.,Buckel, W.,Dobbek, H.
On the ATP-Dependent Activation of the Radical Enzyme (R)-2-Hydroxyisocaproyl-CoA Dehydratase.
Biochemistry, 51:6609-6622, 2012

PubMed Abstract: Members of the 2-hydroxyacyl-CoA dehydratase enzyme family catalyze the β,α-dehydration of various CoA-esters in the fermentation of amino acids by clostridia. Abstraction of the nonacidic β-proton of the 2-hydroxyacyl-CoA compounds is achieved by the reductive generation of ketyl radicals on the substrate, which is initiated by the transfer of an electron at low redox potentials. The highly energetic electron needed on the dehydratase is donated by a [4Fe-4S] cluster containing ATPase, termed activator. We investigated the activator of the 2-hydroxyisocaproyl-CoA dehydratase from Clostridium difficile. The activator is a homodimeric protein structurally related to acetate and sugar kinases, Hsc70 and actin, and has a [4Fe-4S] cluster bound in the dimer interface. The crystal structures of the Mg-ADP, Mg-ADPNP, and nucleotide-free states of the reduced activator have been solved at 1.6-3.0 Å resolution, allowing us to define the position of Mg(2+) and water molecules in the vicinity of the nucleotides and the [4Fe-4S] cluster. The structures reveal redox- and nucleotide dependent changes agreeing with the modulation of the reduction potential of the [4Fe-4S] cluster by conformational changes. We also investigated the propensity of the activator to form a complex with its cognate dehydratase in the presence of Mg-ADP and Mg-ADPNP and together with the structural data present a refined mechanistic scheme for the ATP-dependent electron transfer between activator and dehydratase.

PubMed: 22827463
DOI: 10.1021/bi300571z

実験手法

X-RAY DIFFRACTION (1.6 Å)