4EE9
Crystal structure of the RBcel1 endo-1,4-glucanase
Summary for 4EE9
| Entry DOI | 10.2210/pdb4ee9/pdb |
| Descriptor | Endoglucanase, 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL (3 entities in total) |
| Functional Keywords | glycosyl hydrolase family 5, cellulase, tim barrel, beta-1, 4-endoglucanase, hydrolase |
| Biological source | uncultured bacterium |
| Total number of polymer chains | 1 |
| Total formula weight | 36748.48 |
| Authors | Delsaute, M.,Berlemont, R.,Van Elder, D.,Galleni, M.,Bauvois, C. (deposition date: 2012-03-28, release date: 2013-04-03, Last modification date: 2024-10-30) |
| Primary citation | Delsaute, M.,Berlemont, R.,Dehareng, D.,Van Elder, D.,Galleni, M.,Bauvois, C. Three-dimensional structure of RBcel1, a metagenome-derived psychrotolerant family GH5 endoglucanase. Acta Crystallogr.,Sect.F, 69:828-833, 2013 Cited by PubMed Abstract: RBcel1 is an endoglucanase belonging to glycoside hydrolase family 5 subfamily 5 (GH5_5) that was recently identified from a soil metagenome library from the Antarctic. Unlike its closest structural homologue (Cel5A from Thermoascus aurantiacus), this enzyme was reported to be able to catalyze transglycosylation reactions and has putatively been implicated in the bacterial cellulose-synthesis process. Here, the structure of RBcel1 at 1.4 Å resolution, solved by molecular replacement, is reported. The structure and putative substrate-binding site are described and compared with those of other GH5_5 subfamily members. PubMed: 23908022DOI: 10.1107/S1744309113014565 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.381 Å) |
Structure validation
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