4EE9

Crystal structure of the RBcel1 endo-1,4-glucanase

4EE9 の概要

• エントリーDOI: 10.2210/pdb4ee9/pdb
• 分子名称: Endoglucanase, 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL (3 entities in total)
• 機能のキーワード: glycosyl hydrolase family 5, cellulase, tim barrel, beta-1, 4-endoglucanase, hydrolase
• 由来する生物種: uncultured bacterium
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 36748.48

構造登録者

Delsaute, M.,Berlemont, R.,Van Elder, D.,Galleni, M.,Bauvois, C. (登録日: 2012-03-28, 公開日: 2013-04-03, 最終更新日: 2024-10-30)

主引用文献

Delsaute, M.,Berlemont, R.,Dehareng, D.,Van Elder, D.,Galleni, M.,Bauvois, C.
Three-dimensional structure of RBcel1, a metagenome-derived psychrotolerant family GH5 endoglucanase.
Acta Crystallogr.,Sect.F, 69:828-833, 2013

PubMed Abstract: RBcel1 is an endoglucanase belonging to glycoside hydrolase family 5 subfamily 5 (GH5_5) that was recently identified from a soil metagenome library from the Antarctic. Unlike its closest structural homologue (Cel5A from Thermoascus aurantiacus), this enzyme was reported to be able to catalyze transglycosylation reactions and has putatively been implicated in the bacterial cellulose-synthesis process. Here, the structure of RBcel1 at 1.4 Å resolution, solved by molecular replacement, is reported. The structure and putative substrate-binding site are described and compared with those of other GH5_5 subfamily members.

PubMed: 23908022
DOI: 10.1107/S1744309113014565

実験手法

X-RAY DIFFRACTION (1.381 Å)