4EBZ

Crystal structure of the ectodomain of a receptor like kinase

4EBZ の概要

• エントリーDOI: 10.2210/pdb4ebz/pdb
• 関連するPDBエントリー: 4EBY
• 分子名称: Chitin elicitor receptor kinase 1, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, alpha-D-mannopyranose-(1-3)-[beta-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (5 entities in total)
• 機能のキーワード: pathogen-associated molecular patterns, pattern recognition receptors, chitin elicitor receptor kinase 1, lysm, lysine motif, chitin oligomer, transferase
• 由来する生物種: Arabidopsis thaliana (mouse-ear cress)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 26470.89

構造登録者

Chai, J.,Liu, T.,Han, Z.,She, J,Wang, J. (登録日: 2012-03-26, 公開日: 2012-06-27, 最終更新日: 2024-10-16)

主引用文献

Liu, T.,Liu, Z.,Song, C.,Hu, Y.,Han, Z.,She, J.,Fan, F.,Wang, J.,Jin, C.,Chang, J.,Zhou, J.M.,Chai, J.
Chitin-induced dimerization activates a plant immune receptor.
Science, 336:1160-1164, 2012

PubMed Abstract: Pattern recognition receptors confer plant resistance to pathogen infection by recognizing the conserved pathogen-associated molecular patterns. The cell surface receptor chitin elicitor receptor kinase 1 of Arabidopsis (AtCERK1) directly binds chitin through its lysine motif (LysM)-containing ectodomain (AtCERK1-ECD) to activate immune responses. The crystal structure that we solved of an AtCERK1-ECD complexed with a chitin pentamer reveals that their interaction is primarily mediated by a LysM and three chitin residues. By acting as a bivalent ligand, a chitin octamer induces AtCERK1-ECD dimerization that is inhibited by shorter chitin oligomers. A mutation attenuating chitin-induced AtCERK1-ECD dimerization or formation of nonproductive AtCERK1 dimer by overexpression of AtCERK1-ECD compromises AtCERK1-mediated signaling in plant cells. Together, our data support the notion that chitin-induced AtCERK1 dimerization is critical for its activation.

PubMed: 22654057
DOI: 10.1126/science.1218867

実験手法

X-RAY DIFFRACTION (1.792 Å)