4E3W

Crystal Structure Francisella tularensis histidine acid phosphatase cryoprotected with proline

4E3W の概要

• エントリーDOI: 10.2210/pdb4e3w/pdb
• 関連するPDBエントリー: 4E3U 4E3V 4E3X
• 分子名称: Acid phosphatase, SULFATE ION, PROLINE, ... (4 entities in total)
• 機能のキーワード: hydrolase
• 由来する生物種: Francisella tularensis subsp. holarctica
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 77682.40

構造登録者

Tanner, J.J.,Pemberton, T.A. (登録日: 2012-03-10, 公開日: 2012-07-25, 最終更新日: 2023-09-13)

主引用文献

Pemberton, T.A.,Still, B.R.,Christensen, E.M.,Singh, H.,Srivastava, D.,Tanner, J.J.
Proline: Mother Nature's cryoprotectant applied to protein crystallography.
Acta Crystallogr.,Sect.D, 68:1010-1018, 2012

PubMed Abstract: L-Proline is one of Mother Nature's cryoprotectants. Plants and yeast accumulate proline under freeze-induced stress and the use of proline in the cryopreservation of biological samples is well established. Here, it is shown that L-proline is also a useful cryoprotectant for protein crystallography. Proline was used to prepare crystals of lysozyme, xylose isomerase, histidine acid phosphatase and 1-pyrroline-5-carboxylate dehydrogenase for low-temperature data collection. The crystallization solutions in these test cases included the commonly used precipitants ammonium sulfate, sodium chloride and polyethylene glycol and spanned the pH range 4.6-8.5. Thus, proline is compatible with typical protein-crystallization formulations. The proline concentration needed for cryoprotection of these crystals is in the range 2.0-3.0 M. Complete data sets were collected from the proline-protected crystals. Proline performed as well as traditional cryoprotectants based on the diffraction resolution and data-quality statistics. The structures were refined to assess the binding of proline to these proteins. As observed with traditional cryoprotectants such as glycerol and ethylene glycol, the electron-density maps clearly showed the presence of proline molecules bound to the protein. In two cases, histidine acid phosphatase and 1-pyrroline-5-carboxylate dehydrogenase, proline binds in the active site. It is concluded that L-proline is an effective cryoprotectant for protein crystallography.

PubMed: 22868767
DOI: 10.1107/S0907444912019580

実験手法

X-RAY DIFFRACTION (1.75 Å)