4DWZ

Crystal Structure of Ton_0340

Summary for 4DWZ

• Entry DOI: 10.2210/pdb4dwz/pdb
• Descriptor: Hypothetical protein TON_0340, ZINC ION (3 entities in total)
• Functional Keywords: metal binding protein
• Biological source: Thermococcus onnurineus
• Total number of polymer chains: 6
• Total formula weight: 177130.73

Authors

Lee, S.G.,Lee, K.H.,Cha, S.S.,Oh, B.H. (deposition date: 2012-02-27, release date: 2013-01-16, Last modification date: 2024-03-20)

Primary citation

Cha, S.S.,An, Y.J.,Jeong, C.S.,Kim, M.K.,Lee, S.G.,Lee, K.H.,Oh, B.H.
Experimental phasing using zinc anomalous scattering
Acta Crystallogr.,Sect.D, 68:1253-1258, 2012

PubMed Abstract: Zinc is a suitable metal for anomalous dispersion phasing methods in protein crystallography. Structure determination using zinc anomalous scattering has been almost exclusively limited to proteins with intrinsically bound zinc(s). Here, it is reported that multiple zinc ions can easily be charged onto the surface of proteins with no intrinsic zinc-binding site by using zinc-containing solutions. Zn derivatization of protein surfaces appears to be a largely unnoticed but promising method of protein structure determination.

PubMed: 22948927
DOI: 10.1107/S0907444912024420

Experimental method

X-RAY DIFFRACTION (2.7 Å)