4DT3

Crystal structure of zinc-charged lysozyme

4DT3 の概要

• エントリーDOI: 10.2210/pdb4dt3/pdb
• 分子名称: Lysozyme C, ZINC ION, 1,2-ETHANEDIOL, ... (5 entities in total)
• 機能のキーワード: lysozyme, hydrolase
• 由来する生物種: Gallus gallus (chicken)
• 細胞内の位置: Secreted: P00698
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 14686.98

構造登録者

An, Y.J.,Jeong, C.S.,Cha, S.S. (登録日: 2012-02-20, 公開日: 2012-09-12, 最終更新日: 2024-10-30)

主引用文献

Cha, S.S.,An, Y.J.,Jeong, C.S.,Kim, M.K.,Lee, S.G.,Lee, K.H.,Oh, B.H.
Experimental phasing using zinc anomalous scattering
Acta Crystallogr.,Sect.D, 68:1253-1258, 2012

PubMed Abstract: Zinc is a suitable metal for anomalous dispersion phasing methods in protein crystallography. Structure determination using zinc anomalous scattering has been almost exclusively limited to proteins with intrinsically bound zinc(s). Here, it is reported that multiple zinc ions can easily be charged onto the surface of proteins with no intrinsic zinc-binding site by using zinc-containing solutions. Zn derivatization of protein surfaces appears to be a largely unnoticed but promising method of protein structure determination.

PubMed: 22948927
DOI: 10.1107/S0907444912024420

実験手法

X-RAY DIFFRACTION (1.8 Å)