4DQ4 の概要
| エントリーDOI | 10.2210/pdb4dq4/pdb |
| 関連するPDBエントリー | 3NPO 3NQ3 3NQ9 4DQ3 |
| 分子名称 | Beta-lactoglobulin, LINOLEIC ACID, GLYCEROL, ... (5 entities in total) |
| 機能のキーワード | lipocalin, transport protein, bovine milk |
| 由来する生物種 | Bos taurus (bovine,cow,domestic cattle,domestic cow) |
| 細胞内の位置 | Secreted: P02754 |
| タンパク質・核酸の鎖数 | 1 |
| 化学式量合計 | 18719.78 |
| 構造登録者 | |
| 主引用文献 | Loch, J.I.,Bonarek, P.,Polit, A.,Ries, D.,Dziedzicka-Wasylewska, M.,Lewinski, K. Binding of 18-carbon unsaturated fatty acids to bovine beta-lactoglobulin--structural and thermodynamic studies. Int.J.Biol.Macromol., 57:226-231, 2013 Cited by PubMed Abstract: Binding of 18-carbon unsaturated oleic and linoleic acid to lactoglobulin, the milk protein, has been studied for the first time by isothermal titration calorimetry (ITC) and X-ray crystallography. Crystal structures determined to resolution 2.10 Å have revealed presence of single fatty acid molecule bound in β-barrel, the primary binding site, with carboxyl group hydrogen bonded to Glu62. The aliphatic chain of both ligands is in almost linear conformation and their interactions with the protein are similar to observed in structure of lactoglobulin with stearic acid. The ITC experiments showed that binding of unsaturated fatty acids to LGB is spontaneous and exothermic. The stoichiometry of binding is lower than 1.0, association constant is 9.7 × 10(5)M(-1) and 9.0 × 10(5)M(-1) for oleic and linoleic acid, respectively. Solvent relief seems to be the major contributor to entropic changes upon fatty acid binding to lactoglobulin. PubMed: 23500663DOI: 10.1016/j.ijbiomac.2013.03.021 主引用文献が同じPDBエントリー |
| 実験手法 | X-RAY DIFFRACTION (2.1 Å) |
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