4DOZ

Crystal structure of Pyrococcus furiosus Cmr2 (Cas10)

Summary for 4DOZ

• Entry DOI: 10.2210/pdb4doz/pdb
• Descriptor: Putative uncharacterized protein, ZINC ION (2 entities in total)
• Functional Keywords: cyclase, crispr, zn, antiviral protein
• Biological source: Pyrococcus furiosus
• Cellular location: Cytoplasm: Q8U1S6
• Total number of polymer chains: 1
• Total formula weight: 79544.18

Authors

Zhu, X.,Ye, K. (deposition date: 2012-02-12, release date: 2012-03-21, Last modification date: 2024-03-20)

Primary citation

Zhu, X.,Ye, K.
Crystal structure of Cmr2 suggests a nucleotide cyclase-related enzyme in type III CRISPR-Cas systems
Febs Lett., 586:939-945, 2012

PubMed Abstract: CRISPR RNAs (crRNAs) mediate sequence-specific silencing of invading viruses and plasmids in prokaryotes. The crRNA-Cmr protein complex cleaves complementary RNA. We report the crystal structure of Pyrococcus furiosus Cmr2 (Cas10), a component of this Cmr complex and the signature protein in type III CRISPR systems. The structure reveals a nucleotide cyclase domain with a set of conserved catalytic residues that associates with an unexpected deviant cyclase domain like dimeric cyclases. Additionally, two helical domains resemble the thumb domain of A-family DNA polymerase and Cmr5, respectively. Our results suggest that Cmr2 possesses novel enzymatic activity that remains to be elucidated.

PubMed: 22449983
DOI: 10.1016/j.febslet.2012.02.036

Experimental method

X-RAY DIFFRACTION (3.1 Å)