4DOZ

Crystal structure of Pyrococcus furiosus Cmr2 (Cas10)

4DOZ の概要

• エントリーDOI: 10.2210/pdb4doz/pdb
• 分子名称: Putative uncharacterized protein, ZINC ION (2 entities in total)
• 機能のキーワード: cyclase, crispr, zn, antiviral protein
• 由来する生物種: Pyrococcus furiosus
• 細胞内の位置: Cytoplasm: Q8U1S6
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 79544.18

構造登録者

Zhu, X.,Ye, K. (登録日: 2012-02-12, 公開日: 2012-03-21, 最終更新日: 2024-03-20)

主引用文献

Zhu, X.,Ye, K.
Crystal structure of Cmr2 suggests a nucleotide cyclase-related enzyme in type III CRISPR-Cas systems
Febs Lett., 586:939-945, 2012

PubMed Abstract: CRISPR RNAs (crRNAs) mediate sequence-specific silencing of invading viruses and plasmids in prokaryotes. The crRNA-Cmr protein complex cleaves complementary RNA. We report the crystal structure of Pyrococcus furiosus Cmr2 (Cas10), a component of this Cmr complex and the signature protein in type III CRISPR systems. The structure reveals a nucleotide cyclase domain with a set of conserved catalytic residues that associates with an unexpected deviant cyclase domain like dimeric cyclases. Additionally, two helical domains resemble the thumb domain of A-family DNA polymerase and Cmr5, respectively. Our results suggest that Cmr2 possesses novel enzymatic activity that remains to be elucidated.

PubMed: 22449983
DOI: 10.1016/j.febslet.2012.02.036

実験手法

X-RAY DIFFRACTION (3.1 Å)