4DOD

The structure of Cbescii CelA GH9 module

4DOD の概要

• エントリーDOI: 10.2210/pdb4dod/pdb
• 関連するPDBエントリー: 4DOE
• 分子名称: 1,4-beta-glucanase, 1,4-DIETHYLENE DIOXIDE, CALCIUM ION, ... (7 entities in total)
• 機能のキーワード: cela, gh9, cellulase, (a/a)6 barrel, glycoside hydrolase, hydrolase
• 由来する生物種: Caldicellulosiruptor bescii
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 55435.09

構造登録者

Alahuhta, P.M.,Lunin, V.V. (登録日: 2012-02-09, 公開日: 2013-02-20, 最終更新日: 2023-09-13)

主引用文献

Brunecky, R.,Alahuhta, M.,Xu, Q.,Donohoe, B.S.,Crowley, M.F.,Kataeva, I.A.,Yang, S.J.,Resch, M.G.,Adams, M.W.,Lunin, V.V.,Himmel, M.E.,Bomble, Y.J.
Revealing nature's cellulase diversity: the digestion mechanism of Caldicellulosiruptor bescii CelA.
Science, 342:1513-1516, 2013

PubMed Abstract: Most fungi and bacteria degrade plant cell walls by secreting free, complementary enzymes that hydrolyze cellulose; however, some bacteria use large enzymatic assemblies called cellulosomes, which recruit complementary enzymes to protein scaffolds. The thermophilic bacterium Caldicellulosiruptor bescii uses an intermediate strategy, secreting many free cellulases that contain multiple catalytic domains. One of these, CelA, comprises a glycoside hydrolase family 9 and a family 48 catalytic domain, as well as three type III cellulose-binding modules. In the saccharification of a common cellulose standard, Avicel, CelA outperforms mixtures of commercially relevant exo- and endoglucanases. From transmission electron microscopy studies of cellulose after incubation with CelA, we report morphological features that suggest that CelA not only exploits the common surface ablation mechanism driven by general cellulase processivity, but also excavates extensive cavities into the surface of the substrate. These results suggest that nature's repertoire of cellulose digestion paradigms remain only partially discovered and understood.

PubMed: 24357319
DOI: 10.1126/science.1244273

実験手法

X-RAY DIFFRACTION (1.7 Å)