4DE9

LytR-CPS2A-psr family protein YwtF (TagT) with bound octaprenyl pyrophosphate lipid

4DE9 の概要

• エントリーDOI: 10.2210/pdb4de9/pdb
• 関連するPDBエントリー: 4DE8
• 分子名称: Putative transcriptional regulator ywtF, (2Z,6Z,10Z,14Z,18Z,22E,26E)-3,7,11,15,19,23,27,31-octamethyldotriaconta-2,6,10,14,18,22,26,30-octaen-1-yl trihydrogen diphosphate (3 entities in total)
• 機能のキーワード: possible role in wall techoic acid synthesis, membrane protein
• 由来する生物種: Bacillus subtilis
• 細胞内の位置: Cell membrane; Single-pass type II membrane protein (Potential): Q7WY78
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 32786.31

構造登録者

Eberhardt, A.,Hoyland, C.N.,Vollmer, D.,Bisle, S.,Cleverley, R.M.,Johnsborg, O.,Havarstein, S.,Lewis, R.J.,Vollmer, W. (登録日: 2012-01-20, 公開日: 2012-04-04, 最終更新日: 2024-02-28)

主引用文献

Eberhardt, A.,Hoyland, C.N.,Vollmer, D.,Bisle, S.,Cleverley, R.M.,Johnsborg, O.,Havarstein, L.S.,Lewis, R.J.,Vollmer, W.
Attachment of Capsular Polysaccharide to the Cell Wall in Streptococcus pneumoniae.
Microb Drug Resist, 18:240-255, 2012

PubMed Abstract: Streptococcus pneumoniae protects itself from components of the human immune defense system by a thick polysaccharide capsule, which in most serotypes is covalently attached to the cell wall peptidoglycan. Members of the LytR-Cps2A-Psr (LCP) protein family have recently been implicated in the attachment of anionic polymers to peptidoglycan in Gram-positive bacteria, based on genetic evidence from Bacillus subtilis mutant strains and on the crystal structure of S. pneumoniae Cps2A containing a tightly bound polyprenol (pyro)phosphate lipid. Here, we provide evidence that Cps2A and its two pneumococcal homologs, LytR and Psr, contribute to the maintenance of normal capsule levels and to the retention of the capsular polysaccharide at the cell wall in the capsular type 2 S. pneumoniae strain D39. GFP fusions of all three LCP proteins showed enhanced localization at mid-cell, indicating a role in cell wall growth. Single cps2A or psr mutants produced a reduced amount of capsule. A cps2A lytR double mutant showed greatly impaired growth and cell morphology and lost approximately half of the total capsule material into the culture supernatant. We also present the crystal structure of the B. subtilis LCP protein YwtF and provide crystallographic evidence for the phosphotransferase activity of Cps2A, supporting an enzymatic function in the attachment of capsular polysaccharides to cell wall peptidoglycan.

PubMed: 22432711
DOI: 10.1089/mdr.2011.0232

実験手法

X-RAY DIFFRACTION (1.787 Å)