4DCL
Computationally Designed Self-assembling tetrahedron protein, T308, Crystallized in space group F23
Summary for 4DCL
| Entry DOI | 10.2210/pdb4dcl/pdb |
| Related | 3V4E |
| Descriptor | Putative acetyltransferase SACOL2570 (2 entities in total) |
| Functional Keywords | self assembling tetrahedron design, transferase |
| Biological source | Staphylococcus aureus subsp. aureus |
| Total number of polymer chains | 1 |
| Total formula weight | 22990.97 |
| Authors | Sawaya, M.R.,King, N.P.,Sheffler, W.,Baker, D.,Yeates, T.O. (deposition date: 2012-01-17, release date: 2012-06-06, Last modification date: 2023-09-13) |
| Primary citation | King, N.P.,Sheffler, W.,Sawaya, M.R.,Vollmar, B.S.,Sumida, J.P.,Andre, I.,Gonen, T.,Yeates, T.O.,Baker, D. Computational design of self-assembling protein nanomaterials with atomic level accuracy. Science, 336:1171-1174, 2012 Cited by PubMed Abstract: We describe a general computational method for designing proteins that self-assemble to a desired symmetric architecture. Protein building blocks are docked together symmetrically to identify complementary packing arrangements, and low-energy protein-protein interfaces are then designed between the building blocks in order to drive self-assembly. We used trimeric protein building blocks to design a 24-subunit, 13-nm diameter complex with octahedral symmetry and a 12-subunit, 11-nm diameter complex with tetrahedral symmetry. The designed proteins assembled to the desired oligomeric states in solution, and the crystal structures of the complexes revealed that the resulting materials closely match the design models. The method can be used to design a wide variety of self-assembling protein nanomaterials. PubMed: 22654060DOI: 10.1126/science.1219364 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (3.35 Å) |
Structure validation
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