4DAM

Crystal structure of small single-stranded DNA-binding protein from Streptomyces coelicolor

4DAM の概要

• エントリーDOI: 10.2210/pdb4dam/pdb
• 関連するPDBエントリー: 3EIV
• 分子名称: Single-stranded DNA-binding protein 1 (2 entities in total)
• 機能のキーワード: ob-fold, dna-binding, single-stranded dna, dna binding protein
• 由来する生物種: Streptomyces coelicolor
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 169498.00

構造登録者

Filic, Z.,Herron, P.,Ivic, N.,Luic, M.,Manjasetty, B.A.,Paradzik, T.,Vujaklija, D. (登録日: 2012-01-13, 公開日: 2013-01-16, 最終更新日: 2024-11-20)

主引用文献

Paradzik, T.,Ivic, N.,Filic, Z.,Manjasetty, B.A.,Herron, P.,Luic, M.,Vujaklija, D.
Structure-function relationships of two paralogous single-stranded DNA-binding proteins from Streptomyces coelicolor: implication of SsbB in chromosome segregation during sporulation.
Nucleic Acids Res., 41:3659-3672, 2013

PubMed Abstract: The linear chromosome of Streptomyces coelicolor contains two paralogous ssb genes, ssbA and ssbB. Following mutational analysis, we concluded that ssbA is essential, whereas ssbB plays a key role in chromosome segregation during sporulation. In the ssbB mutant, ∼30% of spores lacked DNA. The two ssb genes were expressed differently; in minimal medium, gene expression was prolonged for both genes and significantly upregulated for ssbB. The ssbA gene is transcribed as part of a polycistronic mRNA from two initiation sites, 163 bp and 75 bp upstream of the rpsF translational start codon. The ssbB gene is transcribed as a monocistronic mRNA, from an unusual promoter region, 73 bp upstream of the AUG codon. Distinctive DNA-binding affinities of single-stranded DNA-binding proteins monitored by tryptophan fluorescent quenching and electrophoretic mobility shift were observed. The crystal structure of SsbB at 1.7 Å resolution revealed a common OB-fold, lack of the clamp-like structure conserved in SsbA and previously unpublished S-S bridges between the A/B and C/D subunits. This is the first report of the determination of paralogous single-stranded DNA-binding protein structures from the same organism. Phylogenetic analysis revealed frequent duplication of ssb genes in Actinobacteria, whereas their strong retention suggests that they are involved in important cellular functions.

PubMed: 23393191
DOI: 10.1093/nar/gkt050

実験手法

X-RAY DIFFRACTION (1.7 Å)