4DA7

Crystal structure of the hexameric purine nucleoside phosphorylase from Bacillus subtilis in complex with aciclovir

4DA7 の概要

• エントリーDOI: 10.2210/pdb4da7/pdb
• 関連するPDBエントリー: 4D8V 4D8X 4D8Y 4D98 4D9H 4DA0 4DA6 4DA8 4DAB 4DAE 4DAN 4DAO 4DAR
• 分子名称: Purine nucleoside phosphorylase deoD-type, 9-HYROXYETHOXYMETHYLGUANINE, PHOSPHATE ION, ... (4 entities in total)
• 機能のキーワード: phosphorylase/hydrolase-like, transferase
• 由来する生物種: Bacillus subtilis
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 27882.31

構造登録者

Martins, N.H.,Giuseppe, P.O.,Meza, A.N.,Murakami, M.T. (登録日: 2012-01-12, 公開日: 2012-09-26, 最終更新日: 2024-02-28)

主引用文献

de Giuseppe, P.O.,Martins, N.H.,Meza, A.N.,Dos Santos, C.R.,Pereira, H.D.,Murakami, M.T.
Insights into phosphate cooperativity and influence of substrate modifications on binding and catalysis of hexameric purine nucleoside phosphorylases.
Plos One, 7:e44282-e44282, 2012

PubMed Abstract: The hexameric purine nucleoside phosphorylase from Bacillus subtilis (BsPNP233) displays great potential to produce nucleoside analogues in industry and can be exploited in the development of new anti-tumor gene therapies. In order to provide structural basis for enzyme and substrates rational optimization, aiming at those applications, the present work shows a thorough and detailed structural description of the binding mode of substrates and nucleoside analogues to the active site of the hexameric BsPNP233. Here we report the crystal structure of BsPNP233 in the apo form and in complex with 11 ligands, including clinically relevant compounds. The crystal structure of six ligands (adenine, 2'deoxyguanosine, aciclovir, ganciclovir, 8-bromoguanosine, 6-chloroguanosine) in complex with a hexameric PNP are presented for the first time. Our data showed that free bases adopt alternative conformations in the BsPNP233 active site and indicated that binding of the co-substrate (2'deoxy)ribose 1-phosphate might contribute for stabilizing the bases in a favorable orientation for catalysis. The BsPNP233-adenosine complex revealed that a hydrogen bond between the 5' hydroxyl group of adenosine and Arg(43*) side chain contributes for the ribosyl radical to adopt an unusual C3'-endo conformation. The structures with 6-chloroguanosine and 8-bromoguanosine pointed out that the Cl(6) and Br(8) substrate modifications seem to be detrimental for catalysis and can be explored in the design of inhibitors for hexameric PNPs from pathogens. Our data also corroborated the competitive inhibition mechanism of hexameric PNPs by tubercidin and suggested that the acyclic nucleoside ganciclovir is a better inhibitor for hexameric PNPs than aciclovir. Furthermore, comparative structural analyses indicated that the replacement of Ser(90) by a threonine in the B. cereus hexameric adenosine phosphorylase (Thr(91)) is responsible for the lack of negative cooperativity of phosphate binding in this enzyme.

PubMed: 22957058
DOI: 10.1371/journal.pone.0044282

実験手法

X-RAY DIFFRACTION (2.05 Å)