4D7E
An unprecedented NADPH domain conformation in Lysine Monooxygenase NbtG from Nocardia farcinica
Summary for 4D7E
| Entry DOI | 10.2210/pdb4d7e/pdb |
| Descriptor | L-LYS MONOOXYGENASE, FLAVIN-ADENINE DINUCLEOTIDE (3 entities in total) |
| Functional Keywords | oxidoreductase, lysine hydroxylase, flavin-dependent monooxygenases, n-hydroxylating monooxygenases, siderophore, c4a-hydroperoxyflavin |
| Biological source | NOCARDIA FARCINICA IFM 10152 |
| Total number of polymer chains | 4 |
| Total formula weight | 189525.11 |
| Authors | Binda, C.,Robinson, R.,Keul, N.,Rodriguez, P.,Robinson, H.H.,Mattevi, A.,Sobrado, P. (deposition date: 2014-11-24, release date: 2015-04-01, Last modification date: 2024-05-08) |
| Primary citation | Binda, C.,Robinson, R.M.,Martin Del Campo, J.S.,Keul, N.D.,Rodriguez, P.J.,Robinson, H.H.,Mattevi, A.,Sobrado, P. An Unprecedented Nadph Domain Conformation in Lysine Monooxygenase Nbtg Provides Insights Into Uncoupling of Oxygen Consumption from Substrate Hydroxylation. J.Biol.Chem., 290:12676-, 2015 Cited by PubMed Abstract: N-Hydroxylating monooxygenases are involved in the biosynthesis of iron-chelating hydroxamate-containing siderophores that play a role in microbial virulence. These flavoenzymes catalyze the NADPH- and oxygen-dependent hydroxylation of amines such as those found on the side chains of lysine and ornithine. In this work we report the biochemical and structural characterization of Nocardia farcinica Lys monooxygenase (NbtG), which has similar biochemical properties to mycobacterial homologs. NbtG is also active on d-Lys, although it binds l-Lys with a higher affinity. Differently from the ornithine monooxygenases PvdA, SidA, and KtzI, NbtG can use both NADH and NADPH and is highly uncoupled, producing more superoxide and hydrogen peroxide than hydroxylated Lys. The crystal structure of NbtG solved at 2.4 Å resolution revealed an unexpected protein conformation with a 30° rotation of the NAD(P)H domain with respect to the flavin adenine dinucleotide (FAD) domain that precludes binding of the nicotinamide cofactor. This "occluded" structure may explain the biochemical properties of NbtG, specifically with regard to the substantial uncoupling and limited stabilization of the C4a-hydroperoxyflavin intermediate. Biological implications of these findings are discussed. PubMed: 25802330DOI: 10.1074/JBC.M114.629485 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.4 Å) |
Structure validation
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