4D7C

Monoclinic crystal form of the extracellular olfactomedin domain from gliomedin

Summary for 4D7C

• Entry DOI: 10.2210/pdb4d7c/pdb
• Related: 4D77
• Descriptor: GLIOMEDIN, SODIUM ION (3 entities in total)
• Functional Keywords: signaling protein, myelin, beta-propeller
• Biological source: RATTUS NORVEGICUS (NORWAY RAT)
• Cellular location: Cell membrane ; Single-pass type II membrane protein : Q80WL1
• Total number of polymer chains: 2
• Total formula weight: 64916.98

Authors

Han, H.,Kursula, P. (deposition date: 2014-11-21, release date: 2014-12-24, Last modification date: 2023-12-20)

Primary citation

Han, H.,Kursula, P.
The Olfactomedin Domain from Gliomedin is a Beta-Propeller with Unique Structural Properties.
J.Biol.Chem., 290:3612-, 2015

PubMed Abstract: All members of the olfactomedin (OLF) family have a conserved extracellular OLF domain, for which a structure has not been available. We present here the crystal structure of the OLF domain from gliomedin. Gliomedin is a protein expressed by Schwann cells in peripheral nerves, important for the formation of the nodes of Ranvier. Gliomedin interacts with neuronal cell adhesion molecules, such as neurofascin, but the structural details of the interaction are not known. The structure of the OLF domain presents a five-bladed β-propeller fold with unusual geometric properties. The symmetry of the structure is not 5-fold, but rather reveals a twisted arrangement. The conserved top face of the gliomedin OLF domain is likely to be important for binding to neuronal ligands. Our results provide a structural basis for the functions of gliomedin in Schwann cells, enable the understanding of the role of the gliomedin OLF domain in autoimmune neuropathies, and unravel the locations of human disease-causing mutations in other OLF family members, including myocilin.

PubMed: 25525261
DOI: 10.1074/JBC.M114.627547

Experimental method

X-RAY DIFFRACTION (1.45 Å)